Anticorps Polyclonal de lapin anti-HSP27

• Phare
• Validé par KD/KO

HSP27 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

34

Avis (1)

N° de cat : 18284-1-AP

Synonymes

28 kDa heat shock protein, Heat shock 27 kDa protein, heat shock 27kDa protein 1, Heat shock protein beta 1, HSP 27, Hsp25, HSP27, HSP28, HSPB1, SRP27, Stress responsive protein 27

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Galerie de données de validation

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  WB analysis using 18284-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18284-1-AP (HSP27 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  WB analysis using 18284-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18284-1-AP (HSP27 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 18284-1-AP

  WB result of HSP27 antibody (18284-1-AP; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSP27 transfected HeLa cells.

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  WB analysis of mouse liver using 18284-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 18284-1-AP (HSP27 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of C6 using 18284-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 18284-1-AP (HSP27 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 18284-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18284-1-AP (HSP27 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 18284-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18284-1-AP (HSP27 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 18284-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18284-1-AP (HSP27 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 18284-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18284-1-AP (HSP27 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 18284-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 18284-1-AP (HSP27 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 18284-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 18284-1-AP (HSP27 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 18284-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18284-1-AP (HSP27 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 18284-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18284-1-AP (HSP27 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 18284-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 18284-1-AP (HSP27 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of MCF-7 using 18284-1-AP

  Immunofluorescent analysis of MCF-7 cells, using HSPB1 antibody 18284-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HeLa using 18284-1-AP

  Immunofluorescent analysis of Hela cells, using HSPB1 antibody 18284-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  FC experiment of MCF-7 using 18284-1-AP

  1X10^6 MCF-7 cells were intracellularly stained with 0.5 ug Anti-Human HSP27 (18284-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules C6, cellules HeLa, cellules HepG2, cellules U2OS, tissu hépatique de souris
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu de cancer du foie humain, tissu de gliome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa, cellules MCF-7
• Résultats positifs en cytométrie: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 26 publications below
• IHC: See 4 publications below
• IF: See 4 publications below
• IP: See 1 publications below
• ELISA: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

18284-1-AP cible HSP27 dans les applications de WB, IP, IHC, IF, FC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, souris, fish
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HSP27 Protéine recombinante Ag13161
• Nom complet: heat shock 27kDa protein 1
• Masse moléculaire calculée: 19-23 kDa
• Poids moléculaire observé: 27 kDa
• Numéro d’acquisition GenBank: BC012768
• Symbole du gène: HSPB1
• Identification du gène (NCBI): 3315
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HSPB1, also known as heat shock protein 27 (HSP27), belongs to the small heat shock protein family which is induced in response to environmental challenges or/and developmental transitions. It is also an anti-apoptotic protein that plays crucial roles in tumorigenesis and cell survival and is reported to be an independent prognosis marker for cancer. Recently HSPB1 has been found to be a valuable marker for melanoma. In addition to the predicted 27 kDa, an extra 50-55 kDa representing dimeric form of HSPB1 may also be observed (PMID: 21353161). It's notable that mouse HSP27 is also named HSP25 and has the predicted MW between 19-23 kDa.

Publications

Species	Application	Title
human	IF	Allergy; ACE2 downregulation in olfactory mucosa: Eosinophilic rhinosinusitis as COVID-19 protective factor?; Authors - Concepció Marin
		Autophagy; ATG4B/autophagin-1 regulates intestinal homeostasis and protects mice from experimental colitis.; Authors - Cabrera Sandra S
rat	IF	J Cell Biol; Human myocytes are protected from titin aggregation-induced stiffening by small heat shock proteins.; Authors - Sebastian Kötter
human	WB,CoIP	Cell Rep; Analysis of proteome-wide degradation dynamics in ALS SOD1 iPSC-derived patient neurons reveals disrupted VCP homeostasis; Authors - Konstantinos Tsioras
human	WB	Antioxid Redox Signal; Peroxiredoxin 6 is a crucial factor in the initial step of mitochondrial clearance and is upstream of the PINK1-Parkin pathway.; Authors - Shuaipeng Ma
human	WB,IHC	CNS Neurosci Ther; FHOD1 is upregulated in glioma cells and attenuates ferroptosis of glioma cells by targeting HSPB1 signaling; Authors - Fan Zhang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tom (Verified Customer) (09-28-2020)

HEK293T lysate (10ug) ran on 4-20% gradient gel. Blocking in 5% BSA for 1 hour, followed by HSP27 (1:1000 in block) overnight at 4 degrees. Goat anti-rabbit HRP (1:10,000) for 1 hour in block.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293

HSP27 Antibody Western Blot validation (1:1000 dilution) in HEK293 (Cat no:18284-1-AP)

Produits associés

Foxp3 / Transcription Factor Staining Buffer Kit

PF00011