Anticorps Monoclonal anti-HSP27

• Phare
• Validé par KD/KO

HSP27 Monoclonal Antibody for WB, IHC, IF, FC (Intra), ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, poisson-zèbre, rat, souris

Applications

WB, IHC, IF, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(3)

CloneNo.

3E7A1

N° de cat : 66767-1-Ig

Synonymes

HSPB1, 3E7A1, CMT2F, Estrogen-regulated 24 kDa protein, HMN2B

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Galerie de données de validation

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  WB analysis using 66767-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66767-1-Ig (HSPB1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66767-1-Ig

  WB result of HSP27 antibody (66767-1-Ig; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSP27 transfected HeLa cells.

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  WB analysis of HSC-T6 using 66767-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66767-1-Ig (HSPB1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 66767-1-Ig

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 66767-1-Ig (HSPB1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 66767-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66767-1-Ig (HSPB1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 66767-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66767-1-Ig (HSP27 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 66767-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using HSP27 antibody (66767-1-Ig, Clone: 3E7A1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of zebrafish retina using 66767-1-Ig

  Antibody staining on tissue sections of the zebrafish retina at 5 days post fertilisation (dpf) for Heatshock protein 27 (Hsp27;magenta); (#66767-1-Ig) Dilution 1:200. Tissue fixed overnight in 4% PFA; DAPI, blue. Data generated by Natalia Jaroszynska in Professor Ryan MacDonald's lab, University College London, UK.

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  FC experiment of HepG2 using 66767-1-Ig

  1X10^6 HepG2 cells were intracellularly stained with 0.5 ug Anti-Human HSP27 (66767-1-Ig, Clone:3E7A1) (red) labeled with FlexAble CoraLite® Plus 555 Antibody Labeling Kit for Mouse IgG1 (KFA022), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HCT 116, cellules A549, cellules HeLa, cellules HepG2, cellules HSC-T6, cellules HuH-7, cellules U2OS, tissu hépatique de souris
• Résultats positifs en IHC: tissu de gliome humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: zebrafish retina, cellules MCF-7
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:20000-1:100000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

66767-1-Ig cible HSP27 dans les applications de WB, IHC, IF, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, poisson-zèbre, rat, souris

• Réactivité: Humain, poisson-zèbre, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: HSP27 Protéine recombinante Ag27859
• Nom complet: heat shock 27kDa protein 1
• Masse moléculaire calculée: 19-23 kDa
• Poids moléculaire observé: 27 kDa
• Numéro d’acquisition GenBank: BC012768
• Symbole du gène: HSPB1
• Identification du gène (NCBI): 3315
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HSPB1, also known as heat shock protein 27 (Hsp27), belongs to the small heat shock protein family which is induced in response to environmental challenges or/and developmental transitions. It is also an anti-apoptotic protein that plays crucial roles in tumorigenesis and cell survival and is reported to be an independent prognosis marker for cancer. Recently HSPB1 has been found to be a valuable marker for melanoma. In addition to the predicted 27 kDa, an extra 50-55 kDa representing dimeric form of HSPB1 may also be observed (21353161).

Publications

Species	Application	Title
		Hum Reprod; T-cadherin inhibits invasion and migration of endometrial stromal cells in endometriosis.; Authors - Qinsheng Lu
human	IF	Oncol Rep; SUMOylation of HSP27 regulates PKM2 to promote esophageal squamous cell carcinoma progression.; Authors - Xiao Zhang
mouse,human	WB,IF	Sci Adv; Reactive astrocytes secrete the chaperone HSPB1 to mediate neuroprotection; Authors - Fangjia Yang

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