Anticorps Recombinant de lapin anti-Phospho-HSP27 (Ser15)

Phospho-HSP27 (Ser15) Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2D4

N° de cat : 83332-3-PBS

Synonymes

HSP27, HSPB1, Hsp25, HMN2B, Estrogen-regulated 24 kDa protein

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Galerie de données de validation

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  WB analysis using 83332-3-RR (same clone as 83332-3-PBS)

  Non-treated HeLa cells, and anisomycin treated HeLa cells were subjected to SDS PAGE followed by western blot with 83332-3-RR (Phospho-HSP27 (Ser15) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin(66009-1-Ig) antibody as loading control. This data was developed using the same antibody clone with 83332-3-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83332-3-RR (same clone as 83332-3-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed Anisomycin treated HeLa cells using Phospho-HSP27 (Ser15) antibody (83332-3-RR, Clone: 2D4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83332-3-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83332-3-RR (same clone as 83332-3-PBS)

  1X10^6 HeLa cells untreated (dashed lines) or treated with λ phosphatase which intracellularly stained with 0.13 ug Phospho-HSP27 (Ser15) Recombinant antibody (83332-3-RR, Clone:2D4) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 83332-3-PBS in a different storage buffer formulation.

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Informations sur le produit

83332-3-PBS cible Phospho-HSP27 (Ser15) dans les applications de WB, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: heat shock 27kDa protein 1
• Masse moléculaire calculée: 19-23 kDa
• Poids moléculaire observé: 27 kDa
• Numéro d’acquisition GenBank: BC012768
• Symbole du gène: HSPB1
• Identification du gène (NCBI): 3315
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

HSPB1, also named as HSP27, belongs to the mammalian small HSP family widely expressed in cells including the cytoplasm and nucleus. It has been reported that HSPB1 plays a key role in stress resistance, actin organization and numerous biological processes. HSPB1 can be phosphorylated in different stimulation such as cytokines, growth factors and peptide hormones on the site of Ser15, Ser78 and Ser82 by MAPKAP2 kinase. The phosphorylation of HSPB1 leads to a decrease in the size of HSPB1 oligomers. HSPB1 is associated with amyotrophic lateral sclerosis (ALS) (PMID: 8542580, 11960785, 10383393, 32231288)

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