Anticorps Monoclonal anti-HSPH1

HSPH1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3H5A5

N° de cat : 66723-1-PBS

Synonymes

Antigen NY CO 25, DKFZp686M05240, Heat shock 110 kDa protein, Heat shock protein 105 kDa, HSP105, HSP105A, HSP105B, HSP110, HSPH1, KIAA0201, NY CO 25

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Galerie de données de validation

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  WB analysis using 66723-1-Ig (same clone as 66723-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66723-1-Ig (HSPH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66723-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 66723-1-Ig (same clone as 66723-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66723-1-Ig (HSPH1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66723-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 66723-1-Ig (same clone as 66723-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66723-1-Ig (HSPH1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66723-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66723-1-Ig (same clone as 66723-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66723-1-Ig (HSPH1 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66723-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66723-1-PBS cible HSPH1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: HSPH1 Protéine recombinante Ag27521
• Nom complet: heat shock 105kDa/110kDa protein 1
• Masse moléculaire calculée: 858 aa, 97 kDa
• Poids moléculaire observé: 110 kDa
• Numéro d’acquisition GenBank: BC037553
• Symbole du gène: HSPH1
• Identification du gène (NCBI): 10808
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

HSP105, also known as HSP110 or HSPH1, belongs to the heat shock protein (HSP) family. Human HSP105 is a high-molecular-weight chaperone protein expressed at constitutively low levels as a cytoplasmic α-isoform and as an inducible nuclear β-isoform on exposure to various forms of stress. HSP105 is constitutively overexpressed in several solid tumors, including melanoma, breast, thyroid, and gastroenteric cancers, and exerts antiapoptotic functions. Recently HSP105 has been identified as a novel candidate biomarker of lymphoma aggressiveness. Western blot analysis using this antibody detected the phosphorylated protein around 110 kDa in various lysates.

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