Anticorps Polyclonal de lapin anti-HTRA2

• Phare
• Validé par KD/KO

HTRA2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, singe, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 15775-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of HeLa using 15775-1-AP (same clone as 15775-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 15775-1-AP (HTRA2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15775-1-PBS in a different storage buffer formulation.

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  WB analysis of human heart using 15775-1-AP (same clone as 15775-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 15775-1-AP (HTRA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15775-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse skeletal muscle using 15775-1-AP (same clone as 15775-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15775-1-AP (HTRA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15775-1-PBS in a different storage buffer formulation.

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  WB analysis of Raji using 15775-1-AP (same clone as 15775-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 15775-1-AP (HTRA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15775-1-PBS in a different storage buffer formulation.

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  IP experiment of Raji using 15775-1-AP (same clone as 15775-1-PBS)

  IP result of anti-HTRA2 (IP:15775-1-AP, 4ug; Detection:15775-1-AP 1:500) with Raji cells lysate 2000ug. This data was developed using the same antibody clone with 15775-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 15775-1-AP (same clone as 15775-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 15775-1-AP (HTRA2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 15775-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 15775-1-AP (same clone as 15775-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 15775-1-AP (HTRA2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 15775-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 15775-1-AP (same clone as 15775-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15775-1-AP (HTRA2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15775-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 15775-1-AP (same clone as 15775-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15775-1-AP (HTRA2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15775-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human thyroid using 15775-1-AP (same clone as 15775-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid tissue slide using 15775-1-AP (HTRA2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15775-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human thyroid using 15775-1-AP (same clone as 15775-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid tissue slide using 15775-1-AP (HTRA2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15775-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 15775-1-AP (same clone as 15775-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15775-1-AP (HTRA2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15775-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 15775-1-AP (same clone as 15775-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15775-1-AP (HTRA2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15775-1-PBS in a different storage buffer formulation.

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Informations sur le produit

15775-1-PBS cible HTRA2 dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, singe, souris

• Réactivité: Humain, rat, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HTRA2 Protéine recombinante Ag8455
• Nom complet: HtrA serine peptidase 2
• Masse moléculaire calculée: 49 kDa
• Poids moléculaire observé: 36 kDa
• Numéro d’acquisition GenBank: BC000096
• Symbole du gène: HTRA2
• Identification du gène (NCBI): 27429
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

HTRA2(High temperature requirement protein A2) is also named as OMI, PRSS25 and belongs to the peptidase S1B family. HTRA2 normally exists in the mitochondria, can cause MMP3 activation in the cytosol under a cell stress condition, which can ultimately lead to demise of dopaminergic neuronal cells(PMID: 22265821). It inhibits mitochondrial superoxide generation, stabilizes mitochondrial membrane potential, and prevents apoptosis at baseline and in response to extracellular inducers of mitochondrial stress(PMID: 18772386). This protein has 4 isoforms produced by alternative splicing.

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