Anticorps Monoclonal anti-HUS1

HUS1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1E8E9

N° de cat : 67168-1-PBS

Synonymes

Checkpoint protein HUS1, hHUS1, HUS1

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Galerie de données de validation

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  WB analysis using 67168-1-Ig (same clone as 67168-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67168-1-Ig (HUS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67168-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 67168-1-Ig (same clone as 67168-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67168-1-Ig (HUS1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67168-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 67168-1-Ig (same clone as 67168-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67168-1-Ig (HUS1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67168-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67168-1-PBS cible HUS1 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: HUS1 Protéine recombinante Ag28527
• Nom complet: HUS1 checkpoint homolog (S. pombe)
• Masse moléculaire calculée: 32 kDa
• Poids moléculaire observé: 32 kDa
• Numéro d’acquisition GenBank: BC007013
• Symbole du gène: HUS1
• Identification du gène (NCBI): 3364
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Hus1 belongs to the Rad family, form a heterotrimeric clamp with Rad1and Rad9 that acts as a putative sensor for DNA damage. This complex plays an apical role in the DNA damage response, loss of a functional complex affects many downstream checkpoint pathways [PMID: 11340080]. It is also a key complex involved in the activation of the ATR pathway. 9-1-1 forms a ring-shaped heterotrimer, resembling the homotrimeric sliding clamp, proliferating cell nuclear antigen (PCNA) [PMID: 19535328].

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