Anticorps Polyclonal de lapin anti-HUWE1

• Phare
• Validé par KD/KO

HUWE1 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

8

N° de cat : 19430-1-AP

Synonymes

ARF binding protein 1, ARF BP1, HECTH9, HSPC272, HUWE1, Ib772, KIAA0312, KIAA1578, Large structure of UREB1, LASU1, Mcl 1 ubiquitin ligase E3, MULE, URE B1, URE binding protein 1, UREB1

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Galerie de données de validation

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  WB analysis using 19430-1-AP

  Daudi cells were subjected to SDS PAGE followed by western blot with 19430-1-AP (HUWE1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 19430-1-AP

  WB result of HUWE1 antibody (19430-1-AP, 1:1000) with 293 cells by Dr. Emilia Galperin, University of Kentucky.

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  IHC staining of human lung cancer using 19430-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19430-1-AP (HUWE1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 19430-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19430-1-AP (HUWE1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 19430-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 19430-1-AP (HUWE1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 19430-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 19430-1-AP (HUWE1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 19430-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 19430-1-AP (HUWE1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 19430-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 19430-1-AP (HUWE1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules Daudi, cellules HEK-293
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu de cancer du côlon humain, tissu pulmonaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 8 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

19430-1-AP cible HUWE1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HUWE1 Protéine recombinante Ag13763
• Nom complet: HECT, UBA and WWE domain containing 1
• Masse moléculaire calculée: 437 aa, 482 kDa
• Poids moléculaire observé: 482 kDa
• Numéro d’acquisition GenBank: BC002602
• Symbole du gène: HUWE1
• Identification du gène (NCBI): 10075
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HUWE1 encodes a HECT domain ubiquitin ligase which is a large protein (500 kDa) has attracted considerable interest because several and quite disparate substrates have been assigned to this E3. It has a role in regulating Berg-mann glia differentiation and this ubiquitin ligase orchestrates the programming of the neural progenitors that give rise to neurons and glia in the cerebellum. HUWE1 is essential for proliferation of a subset of tumor cells, and negative regulator of TP53 during the colorectal carcinoma progression through the ubiquitination pathway mediated by the HECT domain (PMID:15567145). HUWE1 plays a critical role in lung cancer and increased HUWE1 expression is significantly associated with worse prognosis which suggest that HUWE1 might be a potential target for lung cancer therapy (PMID: 30026863).

Publications

Species	Application	Title
human	WB	Mol Cell; WNK1 is an assembly factor for the human ER membrane protein complex.; Authors - Tino Pleiner
human	WB	Sci Adv; Genomic gain of RRS1 promotes hepatocellular carcinoma through reducing the RPL11-MDM2-p53 signaling.; Authors - Pengbo Cao ; KD Validated
human,mouse	WB	FASEB J; HUWE1 promotes EGFR ubiquitination and degradation to protect against renal tubulointerstitial fibrosis.; Authors - Qian Zhu
human	WB	J Cell Sci; Derlin-1 promotes ubiquitination and degradation of epithelial sodium channel (ENaC).; Authors - Hui You ; KD Validated
human	WB,IP	J Cell Sci; The role of USP7 in the Shoc2-ERK1/2 signaling axis and Noonan-like syndrome with loose anagen hair.; Authors - Patricia Wilson ; KD Validated
human	WB	Biochem Biophys Res Commun; NEDD4L-mediated LTF protein degradation limits ferroptosis.; Authors - Yuan Wang