Anticorps Recombinant de lapin anti-HUWE1

HUWE1 Recombinant Antibody for WB, IHC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242702F10

N° de cat : 85177-3-PBS

Synonymes

242702F10, ARF binding protein 1, ARF BP1, ARF-binding protein 1, ARF-BP1

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Galerie de données de validation

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  WB analysis using 85177-3-RR (same clone as 85177-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85177-3-RR (HUWE1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85177-3-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 85177-3-RR (same clone as 85177-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 85177-3-RR (HUWE1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85177-3-PBS in a different storage buffer formulation.

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  IHC staining of human rectal cancer using 85177-3-RR (same clone as 85177-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 85177-3-RR (HUWE1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85177-3-PBS in a different storage buffer formulation.

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  IHC staining of human rectal cancer using 85177-3-RR (same clone as 85177-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 85177-3-RR (HUWE1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85177-3-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 85177-3-RR (same clone as 85177-3-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug HUWE1 Recombinant antibody (85177-3-RR, Clone:242702F10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 85177-3-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85177-3-RR

  Biolayer interferometry (BLl) kinetic assays of 85177-3-RR against Human HUWE1 were performed. The affinity constant is 26.1 nM.

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Informations sur le produit

85177-3-PBS cible HUWE1 dans les applications de WB, IHC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: HUWE1 Protéine recombinante Ag13763
• Nom complet: HECT, UBA and WWE domain containing 1
• Masse moléculaire calculée: 437 aa, 482 kDa
• Poids moléculaire observé: 482 kDa
• Numéro d’acquisition GenBank: BC002602
• Symbole du gène: HUWE1
• Identification du gène (NCBI): 10075
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

HUWE1 encodes a HECT domain ubiquitin ligase which is a large protein (500 kDa) has attracted considerable interest because several and quite disparate substrates have been assigned to this E3. It has a role in regulating Berg-mann glia differentiation and this ubiquitin ligase orchestrates the programming of the neural progenitors that give rise to neurons and glia in the cerebellum. HUWE1 is essential for proliferation of a subset of tumor cells, and negative regulator of TP53 during the colorectal carcinoma progression through the ubiquitination pathway mediated by the HECT domain (PMID:15567145). HUWE1 plays a critical role in lung cancer and increased HUWE1 expression is significantly associated with worse prognosis which suggest that HUWE1 might be a potential target for lung cancer therapy (PMID: 30026863).

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