Anticorps Monoclonal anti-HVCN1

HVCN1 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1E4C4

N° de cat : 66449-1-PBS

Synonymes

HV1, HVCN1, VSOP

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Galerie de données de validation

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  WB analysis of Raji using 66449-1-Ig (same clone as 66449-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 66449-1-Ig (HVCN1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66449-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66449-1-Ig (same clone as 66449-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66449-1-Ig (HVCN1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66449-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66449-1-Ig (same clone as 66449-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66449-1-Ig (HVCN1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66449-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66449-1-Ig (same clone as 66449-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66449-1-Ig (HVCN1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66449-1-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 66449-1-Ig (same clone as 66449-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using HVCN1 antibody (66449-1-Ig, Clone: 1E4C4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66449-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 66449-1-Ig (same clone as 66449-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using HVCN1 antibody (66449-1-Ig, Clone: 1E4C4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66449-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66449-1-PBS cible HVCN1 dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: HVCN1 Protéine recombinante Ag5350
• Nom complet: hydrogen voltage-gated channel 1
• Masse moléculaire calculée: 273 aa, 32 kDa
• Poids moléculaire observé: 28-35 kDa, 40 kDa, 60 kDa
• Numéro d’acquisition GenBank: BC032672
• Symbole du gène: HVCN1
• Identification du gène (NCBI): 84329
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

HVCN1, also named as VSOP and HV1, Belongs to the hydrogen channel family. HVCN1 mediates the voltage-dependent proton permeability of excitable membranes. It forms a proton-selective channel through which protons may pass in accordance with their electrochemical gradient. Proton efflux, HVCN1 is accompanied by membrane depolarization, facilitates acute production of reactive oxygen species in phagocytosis. HVCN1, the voltage-sensitive proton channel, is present in human sperm and is an important regulator of the functional maturation of sperm. HVCN1 has four isoforms with MW 28-32 kDa or 40 kDa (modification). It has a dimer form with MW 60 kDa.

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