Anticorps Recombinant de lapin anti-HVEM/TNFRSF14

HVEM/TNFRSF14 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC

Conjugaison

Non conjugué

CloneNo.

250728C11

N° de cat : 98362-2-RR

Synonymes

TNFRSF14, ATAR, CD270, Herpes virus entry mediator A, Herpesvirus entry mediator A

---

Galerie de données de validation

• 

  FC experiment of human peripheral blood leukocytes using 98362-2-RR

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human HVEM/TNFRSF14 Rabbit RecAb (98362-2-RR, Clone: 250728C11) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human peripheral blood leukocytes using 98362-2-RR

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human HVEM/TNFRSF14 Rabbit RecAb (98362-2-RR, Clone: 250728C11) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  Affinity and Kinetic Characterization of 98362-2-RR

  Biolayer interferometry (BLl) kinetic assays of 98362-2-RR against Human HVEM/TNFRSF14 were performed. The affinity constant is 2.22 nM.

---

Applications testées

• Résultats positifs en cytométrie: human peripheral blood leukocytes

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98362-2-RR cible HVEM/TNFRSF14 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: HVEM/TNFRSF14 Protéine recombinante Eg1275
• Nom complet: tumor necrosis factor receptor superfamily, member 14 (herpesvirus entry mediator)
• Masse moléculaire calculée: 30 kDa
• Numéro d’acquisition GenBank: BC002794
• Symbole du gène: TNFRSF14
• Identification du gène (NCBI): 8764
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2 - 8°C. Stable for one year after shipment.

Informations générales

The mammalian tumor necrosis factor receptor (TNFR) family consists of 10 cell-surface proteins that regulate development and homeostasis of the immune system. Member 14 of TNFR (TNFRSF14, also named as TR2, ATAR, HVEA, HVEM, LIGHTR) was also identified as a mediator of herpesvirus entry into mammalian cells. The cytoplasmic region of TNFRSF14 bound to several members of the TNFR-associated factor (TRAF) family, namely TRAF1, TRAF2, TRAF3, and TRAF5. Transient transfection into human 293 cells caused marked activation of nuclear factor- B (NF- B), a transcriptional regulator of multiple immunomodulatory and inflammatory genes.

{{ptg:RelatedPrimaryAntibodies}}