Anticorps Polyclonal de lapin anti-HYAL2

• Phare
• Validé par KD/KO

HYAL2 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 15115-1-AP

Synonymes

Hyal 2, HYAL2, Hyaluronidase 2, hyaluronoglucosaminidase 2, LuCa 2, LUCA2, Lung carcinoma protein 2

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Galerie de données de validation

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  WB analysis of Raji using 15115-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 15115-1-AP (HYAL2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 15115-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15115-1-AP (HYAL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15115-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15115-1-AP (HYAL2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 15115-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15115-1-AP (HYAL2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 15115-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15115-1-AP (HYAL2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 15115-1-AP

  Immunofluorescent analysis of Hela cells, using HYAL2 antibody 15115-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  FC experiment of HeLa using 15115-1-AP

  1X10^6 HeLa cells were stained with 0.2ug HYAL2 antibody (15115-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). FITC-Goat anti-Rabbit IgG with dilution 1:100.

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, cellules HeLa, cellules Raji, tissu hépatique de souris
• Résultats positifs en IHC: tissu de cancer du poumon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

15115-1-AP cible HYAL2 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HYAL2 Protéine recombinante Ag7157
• Nom complet: hyaluronoglucosaminidase 2
• Masse moléculaire calculée: 54 kDa
• Poids moléculaire observé: 54-60 kDa
• Numéro d’acquisition GenBank: BC000692
• Symbole du gène: HYAL2
• Identification du gène (NCBI): 8692
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Hyaluronic acid (HA) is a glycosaminoglycan that is believed to have numerous important biologic functions, including modulation of cell proliferation, migration, and differentiation, as well as the regulation of extracellular water and protein homeostasis. It is also an integral structural component of cartilage and other tissues and acts as a lubricant in joints. Hyaluronidases are a family of enzymes that catalyse the degradation of HA. In humans, there are five functional hyaluronidases: HYAL1, HYAL2, HYAL3, HYAL4 and HYAL5 (also known as SPAM1 or PH-20); plus a pseudogene, HYAL6 (also known as HYALP1). HYAL2 is present in many tissues except the adult brain (PMID: 11731268). HYAL2 can be exposed to cell surfaces that bind to the plasma membrane via GPI anchors (PMID: 11731268).

Publications

Species	Application	Title
mouse	WB,IF	Genet Med; Elucidating the clinical spectrum and molecular basis of HYAL2 deficiency.; Authors - James Fasham ; KO Validated
human	IHC,IF,WB	Front Immunol; Complement protein C1q stimulates hyaluronic acid degradation via gC1qR/HABP1/p32 in malignant pleural mesothelioma; Authors - Andrea Balduit
MOUSE	WB	PLoS Genet; PMID: 28081210; Authors - Martina M A Muggenthaler ; KO Validated
mouse	WB	Dermatol Ther; Role of Momordica charantia in preventing the natural aging process of skin and sexual organs in mice.; Authors - Keiichi Hiramoto

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