Anticorps Polyclonal de lapin anti-Haptoglobin

Haptoglobin Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, Lapin, porc, rat

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

N° de cat : 30412-1-AP

Synonymes

HP, Zonulin, HPA1S, HP2 ALPHA 2, HAPT

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Galerie de données de validation

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  WB analysis using 30412-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30412-1-AP (Haptoglobin antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis using 30412-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30412-1-AP (Haptoglobin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of human plasma using 30412-1-AP

  IP result of anti-Haptoglobin (IP:30412-1-AP, 4ug; Detection:30412-1-AP 1:3000) with human plasma lysate 1600 ug.

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Applications testées

• Résultats positifs en WB: rabbit blood tissue, plasma de porc
• Résultats positifs en IP: plasma humain,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

30412-1-AP cible Haptoglobin dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat

• Réactivité: Humain, Lapin, porc, rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: haptoglobin
• Poids moléculaire observé: 36-45 kDa
• Numéro d’acquisition GenBank: BC121125
• Symbole du gène: HP
• Identification du gène (NCBI): 3240
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Haptoglobin is also named as Zonulin and belongs to the peptidase S1 family. Haptoglobin is an alpha-2-glycoprotein synthesized mainly by hepatocytes consisting of both alpha and beta subunits linked by disulfide bonds forming dimers (PMID: 4018023). Haptoglobin is primarily produced in the liver, and when red cells are lysed in vivo, free hemoglobin binds to circulating haptoglobin; the hemoglobin-haptoglobin complex is then degraded by the reticuloendothelial system (PMID: 24809098). Because haptoglobin levels become depleted in the presence of large amounts of free hemoglobin, decreased haptoglobin is a marker of hemolysis. The human haptoglobin (HP) gene exists in two major allelic forms leading to three major genotypes: Hp1-1, Hp2-1, and Hp-2-2, that separate on alkaline starch gel electrophoresis due to different molecular weights (PMID: 17474882). Their properties vary in binding strength, anti-oxidative ability, and rate of clearance. Binding strength is greatest with Hp1-1, but the Hp-2 genotypes can bind a larger number of hemoglobin alpha-beta dimers. Complexes of Hp2-2 and hemoglobin have higher affinity for the CD163 receptor, the monocyte-macrophage receptor that facilitates its clearance. Neutrophils contained a large amount of highly glycosylated Hp (β-chain 45-65 kDa) synthesized in neutrophil precursors and stored in specific granules and a small amount of Hp (β-chain 39 kDa) endocytosed from plasma and stored in secretory vesicles (PMID: 16543473).

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