Anticorps Polyclonal de lapin anti-Hemoglobin Alpha

• Phare
• Validé par KD/KO

Hemoglobin Alpha Polyclonal Antibody for WB, IF-P, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF-P, IP, ELISA

Conjugaison

Non conjugué

N° de cat : 14537-1-PBS

Synonymes

HBA1, Hemoglobin alpha chain, Hemoglobin a, HBA2, Alpha-globin

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Galerie de données de validation

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  WB analysis using 14537-1-AP (same clone as 14537-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14537-1-AP (Hemoglobin Alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14537-1-PBS in a different storage buffer formulation.

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  WB analysis using 14537-1-AP (same clone as 14537-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14537-1-AP (Hemoglobin Alpha antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14537-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse liver using 14537-1-AP (same clone as 14537-1-PBS)

  IP result of anti-Hemoglobin Alpha (IP:14537-1-AP, 4ug; Detection:14537-1-AP 1:500) with mouse liver tissue lysate 4000ug. This data was developed using the same antibody clone with 14537-1-PBS in a different storage buffer formulation.

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  IF Staining of human appendicitis using 14537-1-AP (same clone as 14537-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using Hemoglobin Alpha antibody (14537-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14537-1-PBS in a different storage buffer formulation.

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  IF Staining of human placenta using 14537-1-AP (same clone as 14537-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using Hemoglobin Alpha antibody (14537-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14537-1-PBS in a different storage buffer formulation.

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Informations sur le produit

14537-1-PBS cible Hemoglobin Alpha dans les applications de WB, IF-P, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Hemoglobin Alpha Protéine recombinante Ag6040
• Nom complet: hemoglobin, alpha 1
• Masse moléculaire calculée: 15 kDa
• Poids moléculaire observé: 14-16 kDa, 27 kDa
• Numéro d’acquisition GenBank: BC050661
• Symbole du gène: HBA1
• Identification du gène (NCBI): 3039
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The hemoglobin molecule is a tetramer consisting of two alpha- and two beta-globin-like chains. HBA1 (hemoglobin alpha chain) protein is a alpha-type chain of hemoglobin encoded by two independent genes (HBA1 and HBA2) whose coding sequences are identical. Two alpha chains coupled with two beta chains constitute the adult hemoglobin (HbA or α2β2). HBA1 is also the component of fetal hemoglobin (HbF or α2γ2). This antibody detects a major band around 14-16 kDa in the western blot analysis of heart tissue and K-562 cells. A higher band around 26-27 kDa can also be observed occasionally, which may represents the dimer form of HBA1 (PMID: 20836851). This antibody may cross-react with other hemoglobin chains.

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