Anticorps Monoclonal anti-Hemopexin

Hemopexin Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat

Applications

WB, IHC, IF-P, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3A9D6

N° de cat : 66479-1-PBS

Synonymes

HPX, Beta-1B-glycoprotein, Beta 1B glycoprotein, 3A9D6

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Galerie de données de validation

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  WB analysis of human plasma using 66479-1-Ig (same clone as 66479-1-PBS)

  0.7 μL human plasma was subjected to SDS PAGE followed by western blot with 66479-1-Ig (Hemopexin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66479-1-PBS in a different storage buffer formulation.

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  WB analysis of pig plasma using 66479-1-Ig (same clone as 66479-1-PBS)

  0.7 μL pig plasma was subjected to SDS PAGE followed by western blot with 66479-1-Ig (Hemopexin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66479-1-PBS in a different storage buffer formulation.

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  WB analysis of rat serum using 66479-1-Ig (same clone as 66479-1-PBS)

  0.7 μL rat serum was subjected to SDS PAGE followed by western blot with 66479-1-Ig (Hemopexin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66479-1-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 66479-1-Ig (same clone as 66479-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 66479-1-Ig (Hemopexin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66479-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66479-1-Ig (same clone as 66479-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66479-1-Ig (Hemopexin antibody) at dilution of 1:500 (under 10x lens). This data was developed using the same antibody clone with 66479-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66479-1-Ig (same clone as 66479-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66479-1-Ig (Hemopexin antibody) at dilution of 1:500 (under 40x lens). This data was developed using the same antibody clone with 66479-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 66479-1-Ig (same clone as 66479-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66479-1-Ig (Hemopexin antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66479-1-PBS in a different storage buffer formulation.

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  IF Staining of human liver cancer using 66479-1-Ig (same clone as 66479-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Hemopexin antibody (66479-1-Ig, Clone: 3A9D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66479-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 66479-1-Ig (same clone as 66479-1-PBS)

  1X10^6 HepG2 cells were intracellularly stained with 0.5 ug Anti-Human Hemopexin (66479-1-Ig, Clone:3A9D6) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66479-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66479-1-PBS cible Hemopexin dans les applications de WB, IHC, IF-P, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat

• Réactivité: Humain, porc, rat
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Hemopexin Protéine recombinante Ag8533
• Nom complet: hemopexin
• Masse moléculaire calculée: 254 aa, 29 kDa
• Poids moléculaire observé: 65-75 kDa
• Numéro d’acquisition GenBank: BC005395
• Symbole du gène: Hemopexin
• Identification du gène (NCBI): 3263
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Hemopexin (HPX) is the plasma protein responsible for scavenging heme, thus preventing heme-mediated oxidative stress and heme-bound iron loss. In addition, hemopexin blocks heme activation of immune receptors and vascular inflammatory processes. It is mainly expressed in liver, the synthesis of which is induced after inflammation. Alterations of plasma hemopexin level have been linked to disorders like atherosclerosis and inflammatory diseases.

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