Anticorps Recombinant de lapin anti-Hemopexin

Hemopexin Recombinant Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

250345E5

N° de cat : 86395-3-RR

Synonymes

HPX, Beta 1B glycoprotein, Beta-1B-glycoprotein

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Galerie de données de validation

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  WB analysis of human plasma using 86395-3-RR

  human plasma were subjected to SDS PAGE followed by western blot with 86395-3-RR (HPX antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 86395-3-RR

  human placenta tissue were subjected to SDS PAGE followed by western blot with 86395-3-RR (HPX antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  FC experiment of Transfected CHO using 86395-3-RR

  1x10^6 CHO cells or Transfected CHO cells were intracellularly stained with 0.25 ug Hemopexin Recombinant monoclonal antibody (86395-3-RR, Clone:250345E5) and CoraLite647-conjugated F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: tissu placentaire humain, plasma humain
• Résultats positifs en FC (Intra): Transfected CHO,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

86395-3-RR cible Hemopexin dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Hemopexin Protéine recombinante Eg3082
• Nom complet: hemopexin
• Masse moléculaire calculée: 52 kDa
• Poids moléculaire observé: 65-75 kDa
• Numéro d’acquisition GenBank: NM_000613.2
• Symbole du gène: Hemopexin
• Identification du gène (NCBI): 3263
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Hemopexin (HPX) is the plasma protein responsible for scavenging heme, thus preventing heme-mediated oxidative stress and heme-bound iron loss. In addition, hemopexin blocks heme activation of immune receptors and vascular inflammatory processes. It is mainly expressed in liver, the synthesis of which is induced after inflammation. Alterations of plasma hemopexin level have been linked to disorders like atherosclerosis and inflammatory diseases.

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