Anticorps Recombinant de lapin anti-HER2/ErbB2

HER2/ErbB2 Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

241366F4

N° de cat : 84128-5-RR

Synonymes

Her2 / ErbB2, Her2, Erbb2, EC:2.7.10.1, CD340

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Galerie de données de validation

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  WB analysis of 4T1 using 84128-5-RR

  4T1 cells were subjected to SDS PAGE followed by western blot with 84128-5-RR (Her2 / ErbB2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of 4T1 using 84128-5-RR

  Immunofluorescent analysis of (4% PFA) fixed 4T1 cells using Her2 / ErbB2 antibody (84128-5-RR, Clone: 241366F4 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84128-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84128-5-RR against Mouse Her2 / ErbB2 were performed. The affinity constant is 0.356 nM.

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Applications testées

• Résultats positifs en WB: cellules 4T1,
• Résultats positifs en IF/ICC: cellules 4T1,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84128-5-RR cible HER2/ErbB2 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)
• Masse moléculaire calculée: 139 kDa
• Poids moléculaire observé: 185 kDa
• Numéro d’acquisition GenBank: NM_001003817.1
• Symbole du gène: Erbb2
• Identification du gène (NCBI): 13866
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Her2, or ErbB2, is a member of the EGF receptor family of receptor tyrosine kinases. It has no ligand-binding domain of its own. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signaling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase.

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