Anticorps Monoclonal anti-Hexokinase 2

Hexokinase 2 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2A11C3

N° de cat : 66974-1-PBS

Synonymes

HK2, EC:2.7.1.1, Hexokinase type II, hexokinase2, Hexokinase-2

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Galerie de données de validation

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  WB analysis using 66974-1-Ig (same clone as 66974-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66974-1-Ig (Hexokinase 2 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66974-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 66974-1-Ig (same clone as 66974-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 66974-1-Ig (Hexokinase 2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66974-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66974-1-Ig (same clone as 66974-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Hexokinase 2 antibody (66974-1-Ig, Clone: 2A11C3 ) at dilution of 1:750 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66974-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 66974-1-Ig (same clone as 66974-1-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Hexokinase 2 Monoclonal antibody (66974-1-Ig, Clone:2A11C3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66974-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50448-3

  Cytometric bead array standard curve of MP50448-3, HK2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66974-4-PBS. Detection antibody: 66974-1-PBS. Standard:Ag16895. Range: 0.781-100 ng/mL.

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Informations sur le produit

66974-1-PBS cible Hexokinase 2 dans les applications de WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Hexokinase 2 Protéine recombinante Ag16895
• Nom complet: hexokinase 2
• Masse moléculaire calculée: 102 kDa
• Poids moléculaire observé: 102-120 kDa
• Numéro d’acquisition GenBank: BC021116
• Symbole du gène: Hexokinase 2
• Identification du gène (NCBI): 3099
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Hexokinase 2 (HK2), a rate-limiting enzyme in the first step of glycolysis pathway, expresses at high level in cancer cells compared with normal cells. HK2 provides a new target for cancer therapy due to its pivotal role in tumor tumourigenic and metastatic process. HK1 is constitutively expressed in most mammalian adult tissues. HK2, however, although is abundantly expressed in embryonic tissues, is expressed at high levels only in limited number of adult tissues such as adipose, skeletal, and cardiac muscles. (PMID: 29305912, PMID: 28427443, PMID: 23911236). The antibody also recognizes Hexokinase 1.

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