Anticorps Recombinant de lapin anti-Hif1a

Hif1a Recombinant Antibody for IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

230101B3

N° de cat : 82989-6-PBS

Synonymes

ARNT-interacting protein, HIF-1 alpha, Hif-1a, HIF1alpha, HIF-1-alpha

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Galerie de données de validation

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  IHC staining of mouse kidney using 82989-6-RR (same clone as 82989-6-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 82989-6-RR (Hif1a antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82989-6-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 82989-6-RR (same clone as 82989-6-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 82989-6-RR (Hif1a antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82989-6-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82989-6-RR

  Biolayer interferometry (BLl) kinetic assays of 82989-6-RR against Mouse Hif1a were performed. The affinity constant is 0.163 nM.

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Informations sur le produit

82989-6-PBS cible Hif1a dans les applications de IHC, Indirect ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Hif1a Protéine recombinante Ag33620
• Nom complet: hypoxia inducible factor 1, alpha subunit
• Masse moléculaire calculée: 93 kDa
• Numéro d’acquisition GenBank: NM_001313919
• Symbole du gène: Hif1a
• Identification du gène (NCBI): 15251
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

HIF1a, the major regulator of the cellular responses to hypoxia, consists of an oxygen-sensitive subunit, HIF1 alpha (HIF1A), and an oxygen-insensitive subunit, HIF1 beta (arylhydrocarbon receptor nuclear transporter [ARNT]). Under normal oxygen conditions, HIF1a is continuously produced and destroyed, in a process involving hydroxylation, interaction with von Hippel-Lindau (VHL) protein, polyubiquitylation and subsequent proteasomal degradation. Under hypoxic conditions, hydroxylation is impaired and HIF1a is stabilized. HIF1a localizes in cytoplasm in normoxia, but it can translocate into nuclear in response to hypoxia

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