Anticorps Recombinant de lapin anti-Histone H1.0

Histone H1.0 Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

241147D2

N° de cat : 83993-1-PBS

Synonymes

H1F0, 241147D2, H1 histone family, member 0, H10, H1-0

---

Galerie de données de validation

• 

  WB analysis using 83993-1-RR (same clone as 83993-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83993-1-RR (Histone H1.0 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83993-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 83993-1-RR (same clone as 83993-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83993-1-RR (Histone H1.0 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83993-1-PBS in a different storage buffer formulation.

• 

  Sandwich ELISA standard curve of MP00895-1

  Sandwich ELISA standard curve of MP00895-1, Human Histone H1.0 Recombinant Matched Antibody Pair - PBS only. 83993-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag9982. 83993-1-PBS was HRP conjugated as the detection antibody. Range: 0.937-60 ng/mL

• 

  Sample test of MP00895-1

  Hela cell lysate was measured. The human Histone H1.0 concentration of detected samples was determined to be 385.31 ng/mL (based on a 2.0 mg/mL extract load).

• 

  FC experiment of BxPC-3 using 83993-1-RR (same clone as 83993-1-PBS)

  1x10^6 BxPC-3 cells were intracellularly stained with 0.25 ug Histone H1.0 Recombinant antibody (83993-1-RR, Clone:241147D2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83993-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP00895-1

  Cytometric bead array standard curve of MP00895-1, Histone H1.0 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83993-3-PBS. Detection antibody: 83993-1-PBS. Standard: Ag9982. Range: 0.781-100 ng/mL.

---

Informations sur le produit

83993-1-PBS cible Histone H1.0 dans les applications de WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Histone H1.0 Protéine recombinante Ag9982
• Nom complet: H1 histone family, member 0
• Masse moléculaire calculée: 21 kDa
• Poids moléculaire observé: 32 kDa
• Numéro d’acquisition GenBank: BC000145
• Symbole du gène: Histone H1.0
• Identification du gène (NCBI): 3005
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. Linker histones are involved in forming higher order structure in chromatin and maintaining overall chromatin compaction. The H1F0 histones are found in cells that are in terminal stages of differentiation or that have low rates of cell division.Histone H1.0 (H1F0,H1FV) is a linker histone that is widely expressed in many tissues and almost all vertebrates, unlike some other linker histones. The observed molecular weight of H1F0 is about 32 kDa.

{{ptg:RelatedPrimaryAntibodies}}