Anticorps Recombinant de lapin anti-Histone H1.0

Histone H1.0 Recombinant Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

241147D2

N° de cat : 83993-1-RR

Synonymes

H1F0, H1FV, H1-0, H10, H1 histone family, member 0

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Galerie de données de validation

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  WB analysis using 83993-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83993-1-RR (Histone H1.0 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 83993-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83993-1-RR (Histone H1.0 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  FC experiment of BxPC-3 using 83993-1-RR

  1x10^6 BxPC-3 cells were intracellularly stained with 0.25 ug Histone H1.0 Recombinant antibody (83993-1-RR, Clone:241147D2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A431, cellules C6, cellules NIH/3T3, tissu splénique de rat, tissu testiculaire de souris
• Résultats positifs en FC (Intra): cellules BxPC-3,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83993-1-RR cible Histone H1.0 dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Histone H1.0 Protéine recombinante Ag9982
• Nom complet: H1 histone family, member 0
• Masse moléculaire calculée: 21 kDa
• Poids moléculaire observé: 32 kDa
• Numéro d’acquisition GenBank: BC000145
• Symbole du gène: Histone H1.0
• Identification du gène (NCBI): 3005
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. Linker histones are involved in forming higher order structure in chromatin and maintaining overall chromatin compaction. The H1F0 histones are found in cells that are in terminal stages of differentiation or that have low rates of cell division.Histone H1.0 (H1F0,H1FV) is a linker histone that is widely expressed in many tissues and almost all vertebrates, unlike some other linker histones. The observed molecular weight of H1F0 is about 32 kDa.

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