Anticorps Recombinant de lapin anti-Histone H1.0

Histone H1.0 Recombinant Antibody for IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241147F6

N° de cat : 83993-4-PBS

Synonymes

H1F0, H1FV, H1-0, H10, H1 histone family, member 0

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Galerie de données de validation

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  IHC staining of mouse colon using 83993-4-RR (same clone as 83993-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 83993-4-RR (Histone H1.0 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83993-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 83993-4-RR (same clone as 83993-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 83993-4-RR (Histone H1.0 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83993-4-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 83993-4-RR (same clone as 83993-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Histone H1.0 antibody (83993-4-RR, Clone: 241147F6 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83993-4-PBS in a different storage buffer formulation.

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Informations sur le produit

83993-4-PBS cible Histone H1.0 dans les applications de IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Histone H1.0 Protéine recombinante Ag9982
• Nom complet: H1 histone family, member 0
• Masse moléculaire calculée: 21 kDa
• Numéro d’acquisition GenBank: BC000145
• Symbole du gène: Histone H1.0
• Identification du gène (NCBI): 3005
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. Linker histones are involved in forming higher order structure in chromatin and maintaining overall chromatin compaction. The H1F0 histones are found in cells that are in terminal stages of differentiation or that have low rates of cell division.Histone H1.0 (H1F0,H1FV) is a linker histone that is widely expressed in many tissues and almost all vertebrates, unlike some other linker histones.

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