Anticorps Recombinant de lapin anti-Histone H1.0

Histone H1.0 Recombinant Antibody for IF/ICC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

241147F6

N° de cat : CL488-83993-4

Synonymes

H1F0, 241147F6, H1 histone family, member 0, H10, H1-0

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Galerie de données de validation

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  IF Staining of MCF-7 using CL488-83993-4

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite® Plus 488 Histone H1.0 antibody (CL488-83993-4, Clone: 241147F6 ) at dilution of 1:200, CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en IF/ICC: cellules MCF-7,

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-83993-4 cible Histone H1.0 dans les applications de IF/ICC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Histone H1.0 Protéine recombinante Ag9982
• Nom complet: H1 histone family, member 0
• Masse moléculaire calculée: 21 kDa
• Numéro d’acquisition GenBank: BC000145
• Symbole du gène: Histone H1.0
• Identification du gène (NCBI): 3005
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. Linker histones are involved in forming higher order structure in chromatin and maintaining overall chromatin compaction. The H1F0 histones are found in cells that are in terminal stages of differentiation or that have low rates of cell division.Histone H1.0 (H1F0,H1FV) is a linker histone that is widely expressed in many tissues and almost all vertebrates, unlike some other linker histones.

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