Anticorps Recombinant de lapin anti-Histone H2A.X

Histone H2A.X Recombinant Antibody for WB, IHC, ELISA, ChIP-qPCR

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA, ChIP-qPCR

Conjugaison

Non conjugué

CloneNo.

250032F5

N° de cat : 83307-3-RR

Synonymes

H2AFX, H2A histone family, member X, H2A.X, H2AX, Histone H2A

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Galerie de données de validation

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  WB analysis using 83307-3-RR

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 83307-3-RR (Histone H2A.X antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney(renal cell) Cancer using 83307-3-RR

  Immunohistochemical analysis of paraffin-embedded human kidney(renal cell) Cancer slide using 83307-3-RR (Histone H2A.X antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney(renal cell) Cancer using 83307-3-RR

  Immunohistochemical analysis of paraffin-embedded human kidney(renal cell) Cancer slide using 83307-3-RR (Histone H2A.X antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  ChIP experiment of HEK-293 using 83307-3-RR

  Chromatin was prepared from HEK-293 cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Histone H2A.X (83307-3-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.

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  Affinity and Kinetic Characterization of 83307-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83307-3-RR against Human Histone H2A.X were performed. The affinity constant is 3.46 nM.

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules C6, cellules NIH/3T3
• Résultats positifs en IHC: human kidney(renal cell) Cancer, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en ChIP-qPCR: cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• CHImmunoprécipitation (IP)-QPCR: CHIP-QPCR : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83307-3-RR cible Histone H2A.X dans les applications de WB, IHC, ELISA, ChIP-qPCR et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Histone H2A.X Protéine recombinante Ag1305
• Nom complet: H2A histone family, member X
• Masse moléculaire calculée: 15 kDa
• Poids moléculaire observé: 15-18 kDa, 22 kDa
• Numéro d’acquisition GenBank: BC013416
• Symbole du gène: Histone H2A.X
• Identification du gène (NCBI): 3014
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Histone H2A.X belongs to the histone H2A family, which is synthesized in G1 and S phase. It is involved in nucleosomal organization of chromatin together with other histone proteins, and is specially important for recombination between immunoglobulin switch regions. H2A.X becomes phosphorylated on serine 139 (to form gamma-H2AFX or H2AX139ph) in response to DNA double strand breaks (DSBs) generated by exogenous genotoxic agents and by stalled replication forks, which promotes DNA repair and maintains genomic stability. The calculated molecular weight of H2AX is 15 kDa, but the ubiquitinated H2A.X is about 22 kDa.

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