Anticorps Polyclonal de lapin anti-Histone H2B

Histone H2B Polyclonal Antibody for WB, IHC, IF, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 15857-1-PBS

Synonymes

HIST2H2BE, GL105, H2B, H2B.1, H2B/q

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Galerie de données de validation

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  WB analysis using 15857-1-AP (same clone as 15857-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15857-1-AP (Histone H2B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15857-1-PBS in a different storage buffer formulation.

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  WB analysis using 15857-1-AP (same clone as 15857-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15857-1-AP (Histone H2B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15857-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse pancreas using 15857-1-AP (same clone as 15857-1-PBS)

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 15857-1-AP (Histone H2B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15857-1-PBS in a different storage buffer formulation.

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  WB analysis of rat kidney using 15857-1-AP (same clone as 15857-1-PBS)

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 15857-1-AP (Histone H2B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15857-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 15857-1-AP (same clone as 15857-1-PBS)

  IP result of anti-Histone H2B (IP:15857-1-AP, 4ug; Detection:15857-1-AP 1:500) with HEK-293 cells lysate 2000ug. This data was developed using the same antibody clone with 15857-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 15857-1-AP (same clone as 15857-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15857-1-AP (Histone H2B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15857-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 15857-1-AP (same clone as 15857-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15857-1-AP (Histone H2B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15857-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 15857-1-AP (same clone as 15857-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15857-1-AP (Histone H2B Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15857-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 15857-1-AP (same clone as 15857-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15857-1-AP (Histone H2B Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15857-1-PBS in a different storage buffer formulation.

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  IF Staining using 15857-1-AP (same clone as 15857-1-PBS)

  Mouse tumour sections were labelled with H2B (15857-1-AP, red), Dendra2 (green), and DAPI. The image was credited by Dr. Hae Lin Jang from Harvard Medical School. This data was developed using the same antibody clone with 15857-1-PBS in a different storage buffer formulation.

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Informations sur le produit

15857-1-PBS cible Histone H2B dans les applications de WB, IHC, IF, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Histone H2B Protéine recombinante Ag7811
• Nom complet: histone cluster 2, H2be
• Masse moléculaire calculée: 14 kDa
• Poids moléculaire observé: 14-17 kDa
• Numéro d’acquisition GenBank: BC005827
• Symbole du gène: Histone H2B
• Identification du gène (NCBI): 8349
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Histone H2B is a core component of the nucleosome, the fundamental unit of chromatin. It forms a dimer with Histone H2A, and two H2A-H2B dimers combine with a (H3-H4)₂ tetramer to create the histone octamer. The primary function of this structure is to package DNA into a compact and manageable form. However, H2B is also subject to various post-translational modifications (PTMs), such as ubiquitination, which serve as epigenetic marks to regulate gene expression by altering chromatin accessibility.

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