Anticorps Polyclonal de lapin anti-Histone H2B

Histone H2B Polyclonal Antibody for WB, IF/ICC, ChIP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, ChIP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 27740-1-PBS

Synonymes

H2B3B, HIST3H2BB, H2B-clustered histone 26, H2BC26, H2B.U histone 1

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Galerie de données de validation

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  WB analysis of HEK-293T using 27740-1-AP (same clone as 27740-1-PBS)

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 27740-1-AP (HIST3H2BB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27740-1-PBS in a different storage buffer formulation.

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  WB analysis using 27740-1-AP (same clone as 27740-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 27740-1-AP (HIST3H2BB antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27740-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 27740-1-AP (same clone as 27740-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 27740-1-AP (HIST3H2BB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27740-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 27740-1-AP (same clone as 27740-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using Histone H2B antibody (27740-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 27740-1-PBS in a different storage buffer formulation.

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  ChIP experiment of HEK-293 using 27740-1-AP (same clone as 27740-1-PBS)

  Chromatin was prepared from HEK-293 cells, cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 18 µg of cross-linked chromatin, 5 µg of Histone H2B Antibody (27740-1-AP) or 5 ug of Normal Rabbit IgG (30000-0-AP), and 30 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real time PCR. Primers are located in the first kb of the transcribed region. This data was developed using the same antibody clone with 27740-1-PBS in a different storage buffer formulation.

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Informations sur le produit

27740-1-PBS cible Histone H2B dans les applications de WB, IF/ICC, ChIP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Histone H2B Protéine recombinante Ag26901
• Nom complet: histone cluster 3, H2bb
• Poids moléculaire observé: 15-20 kDa
• Numéro d’acquisition GenBank: BC100855
• Symbole du gène: Histone H2B
• Identification du gène (NCBI): 128312
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. HIST3H2BB is a replication-dependent histone that is a member of the histone H2B family.

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