Anticorps Polyclonal de lapin anti-Histone H3.3

• Phare
• Validé par KD/KO

Histone H3.3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 13754-1-PBS

Synonymes

H3F3A, HistoneH3.3, Histone H3, H3-3B, H3-3A

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Galerie de données de validation

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  WB analysis using 13754-1-AP (same clone as 13754-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13754-1-AP (Histone H3.3 antibody) at dilution of 1:1000 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 13754-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse kidney using 13754-1-AP (same clone as 13754-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13754-1-AP (Histone H3.3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13754-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse lung using 13754-1-AP (same clone as 13754-1-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 13754-1-AP (Histone H3.3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13754-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 13754-1-AP (same clone as 13754-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 13754-1-AP (H3F3A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13754-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 13754-1-AP (same clone as 13754-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13754-1-AP (H3F3A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13754-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse thymus using 13754-1-AP (same clone as 13754-1-PBS)

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 13754-1-AP (H3F3A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13754-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 13754-1-AP (same clone as 13754-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13754-1-AP (Histone H3.3 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13754-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 13754-1-AP (same clone as 13754-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using Histone H3.3 antibody (13754-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 13754-1-PBS in a different storage buffer formulation.

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Informations sur le produit

13754-1-PBS cible Histone H3.3 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Histone H3.3 Protéine recombinante Ag4696
• Nom complet: H3 histone, family 3A
• Masse moléculaire calculée: 136 aa, 15 kDa
• Poids moléculaire observé: 15-17 kDa
• Numéro d’acquisition GenBank: BC038989
• Symbole du gène: Histone H3
• Identification du gène (NCBI): 3020
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Histones are the basic nuclear proteins responsible for the nucleosome structure of the chromosomal fiber. Five classes of histone genes have been reported, some of which are expressed only during S phase, while others are replication independent. The latter are referred to as replacement histones and are expressed in quiescent or terminally differentiated cells. [PMID: 8586426] Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. H3F3A is one replacement histone [PMID:16258499].

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