Anticorps Polyclonal de lapin anti-Histone H3.4

Histone H3.4 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 18064-1-PBS

Synonymes

HIST3H3, H3.4, H3-4, H3FT, H3t

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Galerie de données de validation

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  WB analysis using 18064-1-AP (same clone as 18064-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18064-1-AP (Histone H3.4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18064-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 18064-1-AP (same clone as 18064-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18064-1-AP (Histone H3.4 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18064-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 18064-1-AP (same clone as 18064-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18064-1-AP (Histone H3.4 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18064-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat testis using 18064-1-AP (same clone as 18064-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 18064-1-AP (Histone H3.4 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18064-1-PBS in a different storage buffer formulation.

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Informations sur le produit

18064-1-PBS cible Histone H3.4 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Histone H3.4 Protéine recombinante Ag12671
• Nom complet: histone cluster 3, H3
• Masse moléculaire calculée: 136 aa, 16 kDa
• Poids moléculaire observé: 16 kDa
• Numéro d’acquisition GenBank: BC101837
• Symbole du gène: Histone H3.4
• Identification du gène (NCBI): 8290
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

HIST3H3, also known as H3.1t and H3.4, is a histone H3 protein variant that bears the H3.1 variant hallmark cysteine 96 but includes three extra amino acid substitutions. H3.1t was found to be expressed in primary human spermatocytes at the pachytene stage, similar to the human testis-specific histone H1t variant, where it appears therefore to take part in the process of spermatogenesis (PMID: 21782046).

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