Anticorps Monoclonal anti-Histone H3

Histone H3 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

canin, chien, Humain, Lapin, poisson-zèbre, porc, poulet, rat, souris, Hamster, wheat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications(61)

CloneNo.

1A2A3

N° de cat : 68345-1-Ig

Synonymes

HIST1H3A, 1A2A3, Histone H 3, HistoneH3, Histone-H3

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Galerie de données de validation

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  WB analysis using 68345-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68345-1-Ig (HIST1H3A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 68345-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68345-1-Ig (Histone H3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 68345-1-Ig

  IP result of anti-Histone H3 (IP:68345-1-Ig, 4ug; Detection:68345-1-Ig 1:2000) with HeLa cells lysate 1800 ug.

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  IHC staining of human colon cancer using 68345-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68345-1-Ig (Histone H3 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 68345-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68345-1-Ig (HIST1H3A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 68345-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68345-1-Ig (HIST1H3A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 68345-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using Histone H3 antibody (68345-1-Ig, Clone: 1A2A3 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

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  IF Staining of HeLa using 68345-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Histone H3 antibody (68345-1-Ig, Clone: 1A2A3 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of MCF-7 using 68345-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Histone H3 antibody (68345-1-Ig, Clone: 1A2A3 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of HeLa using 68345-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Histone H3 antibody (68345-1-Ig, Clone: 1A2A3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of MCF-7 using 68345-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Histone H3 antibody (68345-1-Ig, Clone: 1A2A3, labeled with CoraLite647-conjugated AffiniPure F(ab')2 Fragment Goat Anti-Mouse IgG (H+L),SA00014-10, magenta) at dilution of 1:400 and CoraLite Plus 488-conjugated ZO-1 antibody (CL488-21773, green), CHCHD6 antibody (66597-1-Ig, Clone: 2A11E9, labeled with FlexAble CoraLite Plus 555 Antibody Labeling Kit for Mouse IgG2a, KFA042, orange).

• 

  IF Staining of HeLa using 68345-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Histone H3 antibody (68345-1-Ig, Clone: 1A2A3 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), Alpha Tubulin antibody (11224-1-AP, red).

• 

  FC experiment of HepG2 using 68345-1-Ig

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human Histone H3 (68345-1-Ig, Clone:1A2A3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules LNCaP, cellules CHO, cellules HEK-293, cellules HeLa, cellules HSC-T6, cellules Jurkat, cellules K-562, cellules MDCK, cellules NIH/3T3, tissu cérébral de poulet, poisson-zèbre
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu testiculaire de souris, tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MCF-7, cellules A431, cellules HeLa
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 58 publications below
• IF: See 3 publications below

Informations sur le produit

68345-1-Ig cible Histone H3 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons canin, chien, Humain, Lapin, poisson-zèbre, porc, poulet, rat, souris, Hamster, wheat

• Réactivité: canin, chien, Humain, Lapin, poisson-zèbre, porc, poulet, rat, souris, Hamster, wheat
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: histone cluster 1, H3a
• Poids moléculaire observé: 15-17 kDa
• Numéro d’acquisition GenBank: BC066245
• Symbole du gène: HIST1H3A
• Identification du gène (NCBI): 8350
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Histones are small, highly basic proteins that consist of a globular domain with unstructured N- and C-terminal tails protruding from the main structure. Histone H3 is one of the five main histones that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. In addition to their role in DNA compartmentalization, histones also play crucial roles in various biologic processes, including gene expression and regulation, DNA repair, chromatin condensation, cell cycle progression, chromosome segregation, and apoptosis. The ability of histones to regulate chromatin dynamics primarily originates from various posttranslational modifications carried out by histone-modifying enzymes.

Publications

Species	Application	Title
human	WB	Mol Cancer; Exosomal circ_0006896 promotes AML progression via interaction with HDAC1 and restriction of antitumor immunity; Authors - Can Can
	WB	Nucleic Acids Res; The origin recognition complex requires chromatin tethering by a hypervariable intrinsically disordered region that is functionally conserved from sponge to man; Authors - Olubu A Adiji
mouse	WB	Redox Biol; Deficiency of S100 calcium binding protein A9 attenuates vascular dysfunction in aged mice; Authors - Boying Zhao
mouse	WB	Environ Pollut; Chronic exposure to polystyrene microplastics induced LHR reduction and decreased testosterone levels through NF-κB pathway; Authors - Haibo Jin
mouse	WB	Biochim Biophys Acta Mol Basis Dis; ARL11 knockdown alleviates spinal cord injury by inhibiting neuroinflammation and M1 activation of microglia in mice; Authors - Haocong Zhang
human	WB	Ecotoxicol Environ Saf; Role of the USP7/FOXO3A axis in environmentally relevant doses of arsenic-induced lung carcinogenesis: Insights from bioinformatics analysis and model of human epithelial cell malignant transformation; Authors - Xiaoyu Chen

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