Anticorps Monoclonal anti-Histone H3 (C-terminal)

Histone H3 (C-terminal) Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, poisson-zèbre, poulet, rat, souris, wheat

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2G10C3

N° de cat : 68503-1-PBS

Synonymes

HIST1H3A, 2G10C3, Histone-H3, HistoneH3, Histone H3

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Galerie de données de validation

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  WB analysis using 68503-1-Ig (same clone as 68503-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68503-1-Ig (HIST1H3A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68503-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 68503-1-Ig (same clone as 68503-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68503-1-Ig (HIST1H3A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68503-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 68503-1-Ig (same clone as 68503-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68503-1-Ig (HIST1H3A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68503-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68503-1-PBS cible Histone H3 (C-terminal) dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, poisson-zèbre, poulet, rat, souris, wheat

• Réactivité: Humain, poisson-zèbre, poulet, rat, souris, wheat
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: histone cluster 1, H3a
• Poids moléculaire observé: 15 kDa
• Numéro d’acquisition GenBank: BC066245
• Symbole du gène: HIST1H3A
• Identification du gène (NCBI): 8350
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Histones are small, highly basic proteins that consist of a globular domain with unstructured N- and C-terminal tails protruding from the main structure. Histone H3 is one of the five main histones that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. In addition to their role in DNA compartmentalization, histones also play crucial roles in various biologic processes, including gene expression and regulation, DNA repair, chromatin condensation, cell cycle progression, chromosome segregation, and apoptosis. The ability of histones to regulate chromatin dynamics primarily originates from various posttranslational modifications carried out by histone-modifying enzymes.

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