Anticorps Monoclonal anti-HuR/ELAVL1

• Phare
• Validé par KD/KO

HuR/ELAVL1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1A11H2

N° de cat : 66549-1-PBS

Synonymes

ELAV like protein 1, ELAV1, ELAVL1, Hu antigen R, Hua, HuR, MelG

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Galerie de données de validation

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  WB analysis using 66549-1-Ig (same clone as 66549-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66549-1-Ig (HuR/ELAVL1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66549-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66549-1-Ig (same clone as 66549-1-PBS)

  WB result of HuR/ELAVL1 antibody (66549-1-Ig; 1:120000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HuR transfected HEK-293 cells. This data was developed using the same antibody clone with 66549-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66549-1-Ig (same clone as 66549-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66549-1-Ig (HuR/ELAVL1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66549-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66549-1-Ig (same clone as 66549-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66549-1-Ig (HuR antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66549-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 66549-1-Ig (same clone as 66549-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66549-1-Ig (HuR antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66549-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66549-1-Ig (same clone as 66549-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66549-1-Ig (HuR antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66549-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 66549-1-Ig (same clone as 66549-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66549-1-Ig (HuR antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66549-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66549-1-Ig (same clone as 66549-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HuR/ELAVL1 antibody (66549-1-Ig, Clone: 1A11H2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66549-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66549-1-PBS cible HuR/ELAVL1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: HuR/ELAVL1 Protéine recombinante Ag2249
• Nom complet: ELAV (embryonic lethal, abnormal vision, Drosophila)-like 1 (Hu antigen R)
• Masse moléculaire calculée: 326 aa, 36 kDa
• Poids moléculaire observé: 34-37 kDa
• Numéro d’acquisition GenBank: BC003376
• Symbole du gène: HuR/ELAVL1
• Identification du gène (NCBI): 1994
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ELAVL1, also named as HUR, belongs to the RRM elav family. It is involved in 3'-UTR ARE-mediated MYC stabilization. ELAVL1 binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, ELAVL1 binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA and AUUUUUA motifs.

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