Anticorps Polyclonal de lapin anti-Huntingtin

Huntingtin Polyclonal Antibody for IHC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

IHC, IF-P, ELISA

Conjugaison

Non conjugué

Avis (1)

N° de cat : 27206-1-AP

Synonymes

HTT, HD, HD protein, Huntingtin, myristoylated N-terminal fragment, Huntington disease protein

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Galerie de données de validation

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  IHC staining of human cerebellum using 27206-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 27206-1-AP (Huntingtin Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human cerebellum using 27206-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 27206-1-AP (Huntingtin Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human brain using 27206-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 27206-1-AP (Huntingtin Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human brain using 27206-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 27206-1-AP (Huntingtin Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of rat cerebellum using 27206-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat cerebellum tissue using Huntingtin antibody (27206-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), FUS/TLS antibody (68262-1-Ig, Clone: 1B4F8, red). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 27206-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Huntingtin antibody (27206-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en IHC: tissu de cervelet humain, tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu de cervelet de rat, tissu cérébral de souris

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

27206-1-AP cible Huntingtin dans les applications de IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Huntingtin Protéine recombinante Ag25922
• Nom complet: huntingtin
• Masse moléculaire calculée: 348 kDa
• Numéro d’acquisition GenBank: NM_002111
• Symbole du gène: Huntingtin
• Identification du gène (NCBI): 3064
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HTT(huntingtin), also named HD and IT15, belongs to the huntingtin family. HTT may play a role in microtubule-mediated transport or vesicle function. Defects in HTT are the cause of Huntington's disease (HD) which is an autosomal dominant neurodegenerative disorder characterized by involuntary movements (chorea), general motor impairment, psychiatric disorders, and dementia.

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Florian (Verified Customer) (03-22-2024)

Primary antibody was tested for ICC, where no specific signal was observed. The antibody therefore was not able to specifically stain Huntingtin aggregates. We then also proceeded to detection of a WB, which resulted in a strong non-specific band. As the theoretical epitope of this antibody is of great interest for us, we would be very looking forward for improvements in this regard.

• Applications: Western Blot, Immunofluorescence
• Cell Tissue Type: HEK293T

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