Anticorps Monoclonal anti-IA-2/PTPRN

IA-2/PTPRN Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG3

Réactivité testée

Humain, Lapin, porc

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

1D3F5

N° de cat : 66045-1-PBS

Synonymes

IA-2, PTPRN, 1D3F5, ICA 512, ICA512-cleaved cytosolic fragment

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Galerie de données de validation

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  WB analysis using 66045-1-Ig (same clone as 66045-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66045-1-Ig (IA-2/PTPRN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66045-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 66045-1-Ig (same clone as 66045-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 66045-1-Ig (IA-2/PTPRN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66045-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 66045-1-Ig (same clone as 66045-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 66045-1-Ig (IA-2/PTPRN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66045-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 66045-1-Ig (same clone as 66045-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66045-1-Ig (IA-2/PTPRN antibody at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66045-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 66045-1-Ig (same clone as 66045-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66045-1-Ig (IA-2/PTPRN antibody at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66045-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 66045-1-Ig (same clone as 66045-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66045-1-Ig (IA-2/PTPRN antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66045-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 66045-1-Ig (same clone as 66045-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66045-1-Ig (IA-2/PTPRN antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66045-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 66045-1-Ig (same clone as 66045-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66045-1-Ig (IA-2/PTPRN antibody at dilution of 1:400 (under 10x lens). This data was developed using the same antibody clone with 66045-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 66045-1-Ig (same clone as 66045-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66045-1-Ig (IA-2/PTPRN antibody at dilution of 1:400 (under 40x lens). This data was developed using the same antibody clone with 66045-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66045-1-Ig (same clone as 66045-1-PBS)

  Immunofluorescent analysis of HepG2 cells using 66045-1-Ig (IA-2/PTPRN antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG. This data was developed using the same antibody clone with 66045-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66045-1-PBS cible IA-2/PTPRN dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc

• Réactivité: Humain, Lapin, porc
• Hôte / Isotype: Mouse / IgG3
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: IA-2/PTPRN Protéine recombinante Ag17909
• Nom complet: protein tyrosine phosphatase, receptor type, N
• Masse moléculaire calculée: 105 kDa
• Poids moléculaire observé: 60-65 kDa, 106-115 kDa
• Numéro d’acquisition GenBank: BC007713
• Symbole du gène: IA-2/PTPRN
• Identification du gène (NCBI): 5798
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

IA-2/PTPRN is a member of the protein tyrosine phosphatase (PTP) family, which contains a transmembrane region, an intracellular PTP-like domain and an extracellular N-terminus. Experiments found that IA-2/PTPRN localizes to secretory granules and is exclusively expressed in neuroendocrine cells (including pancreatic islets cell). IA-2/PTPRNwas found to be a major autoantigen in insulin-dependent diabetes mellitus and the detection of autoantibodies against IA-2/PTPRN is commonly used as a diabetes diagnosis marker.

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