Anticorps Polyclonal de lapin anti-IARS2

• Phare
• Validé par KD/KO

IARS2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 17170-1-AP

Synonymes

IARS2, IleRS, Isoleucine tRNA ligase

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Galerie de données de validation

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  WB analysis using 17170-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17170-1-AP (IARS2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 17170-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17170-1-AP (IARS2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 17170-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 17170-1-AP (IARS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 17170-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17170-1-AP (IARS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 17170-1-AP

  IP Result of anti-IARS2 (IP:17170-1-AP, 4ug; Detection:17170-1-AP 1:800) with HeLa cells lysate 4000ug.

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  IP experiment of Jurkat using 17170-1-AP

  IP Result of anti-IARS2 (IP:17170-1-AP, 4ug; Detection:17170-1-AP 1:800) with Jurkat cells lysate 4000ug.

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  IHC staining of human liver cancer using 17170-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17170-1-AP (IARS2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 17170-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17170-1-AP (IARS2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 17170-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17170-1-AP (IARS2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 17170-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17170-1-AP (IARS2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 17170-1-AP

  Immunofluorescent analysis of HepG2 cells, using IARS2 antibody 17170-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Jurkat, tissu cérébral de souris, tissu pulmonaire de souris
• Résultats positifs en IP: cellules HeLa, cellules Jurkat
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

17170-1-AP cible IARS2 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IARS2 Protéine recombinante Ag10696
• Nom complet: isoleucyl-tRNA synthetase 2, mitochondrial
• Masse moléculaire calculée: 1012 aa, 114 kDa
• Poids moléculaire observé: 114 kDa
• Numéro d’acquisition GenBank: BC010218
• Symbole du gène: IARS2
• Identification du gène (NCBI): 55699
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAS, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of isoleucine-tRNA synthetase exist, a cytoplasmic form and a mitochondrial form. This gene encodes the mitochondrial isoleucine-tRNA synthetase which belongs to the class-I aminoacyl-tRNA synthetase family.

Publications

Species	Application	Title
human	IF, WB	Mol Cell; Mitochondrial Threonyl-tRNA Synthetase TARS2 Is Required for Threonine-Sensitive mTORC1 Activation.; Authors - Sung-Hoon Kim
human	WB	Discov Oncol; High dose isoleucine stabilizes nuclear PTEN to suppress the proliferation of lung cancer; Authors - Haiqing Wang
human	WB	Rev Assoc Med Bras (1992); IARS2 regulates proliferation, migration, and angiogenesis of human umbilical vein endothelial cells.; Authors - Yue-Ming Yu
human	WB	Front Oncol; The Oncogene IARS2 Promotes Non-small Cell Lung Cancer Tumorigenesis by Activating the AKT/MTOR Pathway.; Authors - Xin Di ; KD Validated