Anticorps Recombinant de lapin anti-IARS2

IARS2 Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242219G4

N° de cat : 84764-5-PBS

Synonymes

242219G4, EC:6.1.1.5, IleRS, Isoleucine tRNA ligase, Isoleucine--tRNA ligase, mitochondrial

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Galerie de données de validation

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  WB analysis using 84764-5-RR (same clone as 84764-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84764-5-RR (IARS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84764-5-PBS in a different storage buffer formulation.

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  IHC staining of human hepatocellular cancer using 84764-5-RR (same clone as 84764-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human hepatocellular cancer slide using 84764-5-RR (IARS2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84764-5-PBS in a different storage buffer formulation.

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  IHC staining of human hepatocellular cancer using 84764-5-RR (same clone as 84764-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human hepatocellular cancer slide using 84764-5-RR (IARS2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84764-5-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 84764-5-RR (same clone as 84764-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using IARS2 antibody (84764-5-RR, Clone: 242219G4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84764-5-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84764-5-RR (same clone as 84764-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using IARS2 antibody (84764-5-RR, Clone: 242219G4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 84764-5-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 84764-5-RR (same clone as 84764-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using IARS2 antibody (84764-5-RR, Clone: 242219G4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 84764-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84764-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84764-5-RR against Human IARS2 were performed. The affinity constant is 0.154 nM.

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Informations sur le produit

84764-5-PBS cible IARS2 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: IARS2 Protéine recombinante Ag10696
• Nom complet: isoleucyl-tRNA synthetase 2, mitochondrial
• Masse moléculaire calculée: 1012 aa, 114 kDa
• Poids moléculaire observé: 114 kDa
• Numéro d’acquisition GenBank: BC010218
• Symbole du gène: IARS2
• Identification du gène (NCBI): 55699
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAS, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of isoleucine-tRNA synthetase exist, a cytoplasmic form and a mitochondrial form. This gene encodes the mitochondrial isoleucine-tRNA synthetase which belongs to the class-I aminoacyl-tRNA synthetase family.

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