Anticorps Polyclonal de lapin anti-IBA1

IBA1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(14)

N° de cat : 26177-1-AP

Synonymes

AIF1, AIF 1, IBA 1, G1, allograft inflammatory factor 1

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Galerie de données de validation

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  WB analysis of THP-1 using 26177-1-AP

  THP-1 cells were subjected to SDS PAGE followed by western blot with 26177-1-AP (IBA1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of rat brain using 26177-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 26177-1-AP (IBA1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 26177-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 26177-1-AP (IBA1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 26177-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26177-1-AP (IBA1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 26177-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26177-1-AP (IBA1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules THP-1,
• Résultats positifs en IHC: tissu cérébral de rat, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:400-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 4 publications below
• IF: See 7 publications below

Informations sur le produit

26177-1-AP cible IBA1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IBA1 Protéine recombinante Ag24000
• Nom complet: allograft inflammatory factor 1
• Masse moléculaire calculée: 17 kDa
• Numéro d’acquisition GenBank: BC009474
• Symbole du gène: IBA1
• Identification du gène (NCBI): 199
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IBA1 is a 143 amino acid cytoplasmic, inflammation response scaffold protein. It is constitutively expressed in monocytes and macrophages and is known to be involved in macrophage activation. It is a marker of activated macrophage. Expression of IBA1 is up-regulated in activated microglia following facial nerve axotomy, ischemia, and several brain diseases, thereby implicating it in the activated phenotypes of microglia.

Publications

Species	Application	Title
mouse	WB	Alzheimers Res Ther; Circular RNA APP contributes to Alzheimer's disease pathogenesis by modulating microglial polarization via miR-1906/CLIC1 axis; Authors - Deng-Pan Wu
mouse	IF	Fitoterapia; LC-MS/MS analysis of Shenghui decoction component and its effect on learning and memory and neuroprotection in sleep deprivation model mice; Authors - Chun Wen
mouse,rat	WB,IF	Toxicon; Loganin reduces neuroinflammation and promotes functional recovery after spinal cord injury through inhibiting NF-κB/NLRP3 signalling; Authors - Xudong Huang
mouse	WB	Front Pharmacol; Shikimic acid (SA) inhibits neuro-inflammation and exerts neuroprotective effects in an LPS-induced in vitro and in vivo model; Authors - Xueying Bao
rat	IHC	J Pain Res; Rat Model of Neuropathic Pain Induced by Spinal Nerve Ligation: A New Approach via an Oblique Lateral Incision; Authors - Zhihong Cheng
mouse	WB	Brain Res Bull; Induction of tau pathology and motor dysfunction in mice by urinary exosomes from progressive supranuclear palsy patients; Authors - Qiusha Li

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