Anticorps Polyclonal de lapin anti-Icam-1

Icam-1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat, souris et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

17

N° de cat : 16174-1-AP

Synonymes

CD54, ICAM 1, Icam1, Icam-1, Ly 47, MALA 2

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Galerie de données de validation

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  WB analysis of rat heart using 16174-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 16174-1-AP (Icam-1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of 4T1 using 16174-1-AP

  IP result of anti-Icam-1 (IP:16174-1-AP, 4ug; Detection:16174-1-AP 1:300) with 4T1 cells lysate 2800 ug.

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  IHC staining of mouse lung using 16174-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 16174-1-AP (Icam-1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 16174-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 16174-1-AP (Icam-1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of 4T1 using 16174-1-AP

  Immunofluorescent analysis 4T1 cells using 16174-1-AP (Icam-1 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.

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  IF Staining of 4T1 using 16174-1-AP

  Immunofluorescent analysis of 4T1 cells using 16174-1-AP (Icam-1 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.

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Applications testées

• Résultats positifs en WB: tissu cardiaque de rat
• Résultats positifs en IP: cellules 4T1
• Résultats positifs en IHC: tissu pulmonaire de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules 4T1

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 13 publications below
• IHC: See 2 publications below
• IF: See 4 publications below

Informations sur le produit

16174-1-AP cible Icam-1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons rat, souris

• Réactivité: rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: intercellular adhesion molecule 1
• Masse moléculaire calculée: 59 kDa
• Poids moléculaire observé: 85-95 kDa
• Numéro d’acquisition GenBank: NM_010493
• Symbole du gène: Icam1
• Identification du gène (NCBI): 15894
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Where is ICAM-1 expressed?

Intercellular Adhesion Molecule 1 (ICAM-1), also known as Cluster of Differentiation 54 (CD54) is a transmembrane glycoprotein constitutively expressed at low levels in endothelial cells, pericytes and on some lymphocytes and monocytes¹. It is located at the cytoplasmic membrane, with a large extracellular region of mainly hydrophobic amino acids joined to a small transmembrane region and a cytoplasmic tail. It has a molecular weight of 75 to 115 kDa depending on the level of glycosylation.

 What is the function of ICAM-1?

ICAM-1 is important in both innate and adaptive immune responses as an adhesion molecule. Although it is constitutively expressed, in the presence of pro-inflammatory cytokines such as TNFα the endothelial cells are activated and upregulate expression of ICAM-1². In blood vessels lined with endothelial cells, leukocytes that are rolling over the surface are able to bind to ICAM-1 and transmigrate through the endothelial barrier and into the tissue. The initial binding of the leukocytes to ICAM-1 causes a Ca2+ release that initiates endothelial cell contraction and weakening of the intercellular tight junctions^{3, 4}. This protein can be used as an indicator of endothelial activation and of vascular inflammation.

 What is the role of ICAM-1 in disease?

Beyond the role in the immune response, ICAM-1 has also been identified as the target of attachment for the human rhinovirus, the cause of the common cold. Binding of the virus to ICAM-1 causes the viral capsid to uncoat and leads to release of the genetic material⁵.

1.  Hubbard, A. K. & Rothlein, R. Intercellular adhesion molecule-1 (ICAM-1) expression and cell signaling cascades. Free Radic. Biol. Med. 28, 1379-86 (2000).

2. Long, E. O. ICAM-1: getting a grip on leukocyte adhesion. J. Immunol. 186, 5021-3 (2011).

3. Lawson, C. & Wolf, S. ICAM-1 signaling in endothelial cells. (2009).

4. Lyck, R. & Enzmann, G. The physiological roles of ICAM-1 and ICAM-2 in neutrophil migration into tissues. Curr. Opin. Hematol. 22, 53-59 (2015).

5. Xing, L., Casasnovas, J. M. & Cheng, R. H. Structural analysis of human rhinovirus complexed with ICAM-1 reveals the dynamics of receptor-mediated virus uncoating. J. Virol. 77, 6101-7 (2003).

Publications

Species	Application	Title
human,mouse	WB	Adv Sci (Weinh); Astrocyte-Derived Extracellular Vesicular miR-143-3p Dampens Autophagic Degradation of Endothelial Adhesion Molecules and Promotes Neutrophil Transendothelial Migration after Acute Brain Injury; Authors - Xun Wu
mouse,rat	WB	Clin Sci (Lond); Cardioprotective and anti-inflammatory effects of Caveolin 1 in experimental diabetic cardiomyopathy; Authors - Wenyan Gong
human,mouse	WB,IF	J Neuroinflammation; SARS-CoV-2 productively infects human brain microvascular endothelial cells.; Authors - Rui-Cheng Yang
human	WB	J Clin Endocrinol Metab; LncRNA LPAL2/miR-1287-5p/EGFR axis modulates TED derived orbital fibroblast activation through cell adhesion factors.; Authors - Nuo Wang
rat	IHC	Transplantation; The Selective RNA Polymerase I Inhibitor CX-5461 Mitigates Neointimal Remodeling in a Modified Model of Rat Aortic Transplantation.; Authors - Chaochao Dai
mouse	WB	Front Pharmacol; Precise Investigation of the Efficacy of Multicomponent Drugs Against Pneumonia Infected With Influenza Virus.; Authors - Junying Wei