Anticorps Recombinant de lapin anti-ICAM-1/CD54

ICAM-1/CD54 Recombinant Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2E23

N° de cat : 82827-1-PBS

Synonymes

CD54, ICAM-1, MALA-2, Icam1, ICAM 1

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Galerie de données de validation

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  WB analysis using 82827-1-RR (same clone as 82827-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82827-1-RR (ICAM-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82827-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse lung using 82827-1-RR (same clone as 82827-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 82827-1-RR (ICAM-1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82827-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse lung using 82827-1-RR (same clone as 82827-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 82827-1-RR (ICAM-1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82827-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 82827-1-RR (same clone as 82827-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 82827-1-RR (ICAM-1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82827-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 82827-1-RR (same clone as 82827-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 82827-1-RR (ICAM-1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82827-1-PBS in a different storage buffer formulation.

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Informations sur le produit

82827-1-PBS cible ICAM-1/CD54 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons rat, souris

• Réactivité: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: intercellular adhesion molecule 1
• Masse moléculaire calculée: 59 kDa
• Poids moléculaire observé: 85-110 kDa
• Numéro d’acquisition GenBank: NM_010493
• Symbole du gène: ICAM-1
• Identification du gène (NCBI): 15894
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ICAM-1 (CD54) is a transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues (PMID: 19307690). ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin (PMID: 3086451; 9694714; 15979056). It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum (PMID: 2566624; 2538244; 2475784).

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