Anticorps Recombinant de lapin anti-ICAM-1/CD54

ICAM-1/CD54 Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

230342A2

N° de cat : 83069-6-PBS

Synonymes

CD54, ICAM-1, ICAM1, ICAM 1, BB2

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Galerie de données de validation

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  WB analysis using 83069-6-RR (same clone as 83069-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83069-6-RR (ICAM-1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83069-6-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83069-6-RR (same clone as 83069-6-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ICAM-1 antibody (83069-6-RR, Clone: 230342A2 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83069-6-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 83069-6-RR (same clone as 83069-6-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ICAM-1 antibody (83069-6-RR, Clone: 230342A2 ) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83069-6-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83069-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83069-6-RR against Human ICAM-1 were performed. The affinity constant is 0.271 nM.

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Informations sur le produit

83069-6-PBS cible ICAM-1/CD54 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: intercellular adhesion molecule 1
• Masse moléculaire calculée: 90 kDa
• Poids moléculaire observé: 85-95 kDa
• Numéro d’acquisition GenBank: BC015969
• Symbole du gène: ICAM-1
• Identification du gène (NCBI): 3383
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ICAM-1 (CD54) is a 90-kDa transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues (PMID: 19307690). ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin (PMID: 3086451; 9694714; 15979056). It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum (PMID: 2566624; 2538244; 2475784).

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