Anticorps Recombinant de lapin anti-ICAM-1/CD54

ICAM-1/CD54 Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

240725E10

N° de cat : 83696-4-PBS

Synonymes

CD54, ICAM-1, ICAM-12, Icam1, 240725E10

---

Galerie de données de validation

• 

  Sandwich ELISA standard curve of MP00678-2

  Sandwich ELISA standard curve of MP00678-2, Rat ICAM-1/CD54 Recombinant Matched Antibody Pair - PBS only. 83696-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1025. 83696-2-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/mL.

• 

  Sample test of MP00678-2

  Serum of sixteen rats was measured. The ICAM-1/CD54 concentration of detected samples was determined to be 15.3 ng/mL with a range of 12.9 - 20.5 ng/mL.

• 

  Sample test of MP00678-2

  Rat heart conditioned media (1 heart, 1-2 mm pieces) was cultured for 4 days in 30 mL of RPMI supplemented with 10% fetal bovine serum and stimulated with 2.5 ng/mL LPS. The mean ICAM-1/CD54 concentration was determined to be 2,606.5 pg/mL in stimulated heart supernatant.
  Spleens from rats were removed, rinsed in PBS, and kept on ice. The tissue was homogenized using a tissue homogenizer and seeded into media containing DMEM supplemented with 10% fetal bovine serum, 2 mM L-glutamine, 100 U/mL penicillin, 100 μg/mL of streptomycin sulfate, and 50 ng/mL gentamicin solution. Cells were culture stimulated with 50 ng/mL of recombinant rat IL-2 and 50 ng/ml PHA for 3 days. The mean ICAM-1/CD54 concentration was determined to be 423.9 pg/mL in stimulated spleen supernatant.

• 

  Cytometric bead array standard curve of MP00678-2

  Cytometric bead array standard curve of MP00678-2, RAT ICAM-1/CD54 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83696-4-PBS. Detection antibody: 83696-2-PBS. Standard: Eg1025. Range: 0.156-20 ng/mL.

---

Informations sur le produit

83696-4-PBS cible ICAM-1/CD54 dans les applications de Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons rat

• Réactivité: rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: intercellular adhesion molecule 1
• Masse moléculaire calculée: 60 kDa
• Numéro d’acquisition GenBank: NM_012967.1
• Symbole du gène: Icam1
• Identification du gène (NCBI): 25464
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

{{ptg:RelatedPrimaryAntibodies}}