Anticorps Recombinant de lapin anti-ICAM-1/CD54

ICAM-1/CD54 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat

Applications

FC

Conjugaison

Non conjugué

CloneNo.

240725C2

N° de cat : 98060-1-RR

Synonymes

CD54, ICAM-1, ICAM-12, Icam1, 240725C2

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Galerie de données de validation

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  FC experiment of rat splenocytes using 98060-1-RR

  1x10^6 rat splenocytes were surface stained with 0.25 ug Anti-Rat ICAM-1/CD54 Rabbit Recombinant Antibody (98060-1-RR, Clone:240725C2) (red) or isotype control (blue) and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed.

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  Affinity and Kinetic Characterization of 98060-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98060-1-RR against Mouse ICAM-1/CD54 were performed. The affinity constant is 0.347 nM.

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Applications testées

• Résultats positifs en cytométrie: splénocytes de rat

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98060-1-RR cible ICAM-1/CD54 dans les applications de FC et montre une réactivité avec des échantillons rat

• Réactivité: rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: intercellular adhesion molecule 1
• Masse moléculaire calculée: 60 kDa
• Numéro d’acquisition GenBank: NM_012967.1
• Symbole du gène: Icam1
• Identification du gène (NCBI): 25464
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2 - 8°C. Stable for one year after shipment.

Informations générales

ICAM-1 (CD54) is a transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues (PMID: 19307690). ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin (PMID: 3086451; 9694714; 15979056). It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum (PMID: 2566624; 2538244; 2475784).

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