Anticorps Recombinant de lapin anti-ICAM-1/CD54

ICAM-1/CD54 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

FC

Conjugaison

CoraLite® Plus 405 Fluorescent Dye

CloneNo.

240249D10

N° de cat : CL405-98049

Synonymes

CD54, Icam-1, 240249D10, ICAM 1, Icam1

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Galerie de données de validation

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  FC experiment of mouse splenocytes using CL405-98049

  1x10^6 mouse splenocytes were surface stained with CoraLite® Plus 488 Anti-Mouse CD3 (17A2) (CL488-65077, Clone: 17A2), and 0.25 ug CoraLite® Plus 405 Anti-Mouse ICAM-1/CD54 Rabbit Recombinant Antibody (CL405-98049, Clone: 240249D10) or Isotype Control. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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  FC experiment of mouse splenocytes using CL405-98049

  1x10^6 mouse splenocytes were surface stained with 0.25 ug CoraLite® Plus 405 Anti-Mouse ICAM-1/CD54 Rabbit Recombinant Antibody (CL405-98049, Clone:240249D10) (red) or Isotype Control (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: splénocytes de souris

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL405-98049 cible ICAM-1/CD54 dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ICAM-1/CD54 Protéine recombinante Eg0482
• Nom complet: intercellular adhesion molecule 1
• Masse moléculaire calculée: 59 kDa
• Numéro d’acquisition GenBank: NM_010493
• Symbole du gène: ICAM-1
• Identification du gène (NCBI): 15894
• Conjugaison: CoraLite® Plus 405 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 399 nm / 422 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

ICAM-1 (CD54) is a 90-kDa transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues (PMID: 19307690). ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin (PMID: 3086451; 9694714; 15979056). It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum (PMID: 2566624; 2538244; 2475784).

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