Anticorps Recombinant de lapin anti-ICAM-2/CD102

ICAM-2/CD102 Recombinant Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241387F11

N° de cat : 84442-1-PBS

Synonymes

ICAM-2, ICAM2, ICAM 2, CD102, 241387F11

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Galerie de données de validation

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  WB analysis using 84442-1-RR (same clone as 84442-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84442-1-RR (ICAM-2/CD102 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84442-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 84442-1-RR (same clone as 84442-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 84442-1-RR (ICAM-2/CD102 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84442-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse lung using 84442-1-RR (same clone as 84442-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 84442-1-RR (ICAM-2/CD102 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84442-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 84442-1-RR (same clone as 84442-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84442-1-RR (ICAM-2/CD102 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84442-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 84442-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84442-1-RR against Mouse ICAM-2/CD102 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

84442-1-PBS cible ICAM-2/CD102 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: intercellular adhesion molecule 2
• Masse moléculaire calculée: 31kDa
• Poids moléculaire observé: 45-55 kDa
• Numéro d’acquisition GenBank: NM_010494.2
• Symbole du gène: Icam2
• Identification du gène (NCBI): 15896
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ICAM2 is a cell adhesion protein having important roles in cell migration, especially during inflammation when leukocytes cross the endothelium. Initially described as a receptor for lymphocyte function-associated antigen-1 (LFA1), ICAM2 may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance. ICAM2 has six N-linked glycosylation sites at amino acids (asparagines) 47, 82, 105, 153, 178 and 187.

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