Anticorps Polyclonal de lapin anti-ICAM3/CD50

• Phare
• Validé par KD/KO

ICAM3/CD50 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

N° de cat : 17699-1-AP

Synonymes

CDW50, ICAM3, CD50, ICAM 3, ICAM R

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Galerie de données de validation

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  WB analysis of Jurkat using 17699-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17699-1-AP (ICAM3/CD50 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 17699-1-AP

  WB result of ICAM3/CD50 antibody (17699-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ICAM3/CD50 transfected Jurkat cells.

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  WB analysis of Ramos using 17699-1-AP

  Ramos cells were subjected to SDS PAGE followed by western blot with 17699-1-AP (ICAM3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of U-937 using 17699-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 17699-1-AP (ICAM3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 17699-1-AP

  IP result of anti-ICAM3/CD50 (IP:17699-1-AP, 4ug; Detection:17699-1-AP 1:500) with Jurkat cells lysate 4000 ug.

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  IHC staining of human colon using 17699-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 17699-1-AP (ICAM3/CD50 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human colon using 17699-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 17699-1-AP (ICAM3 antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules Ramos, cellules U-937
• Résultats positifs en IP: cellules Jurkat,
• Résultats positifs en IHC: tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2400
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

17699-1-AP cible ICAM3/CD50 dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ICAM3/CD50 Protéine recombinante Ag11955
• Nom complet: intercellular adhesion molecule 3
• Masse moléculaire calculée: 547 aa, 60 kDa
• Poids moléculaire observé: 110-160 kDa, 60 kDa
• Numéro d’acquisition GenBank: BC058903
• Symbole du gène: ICAM3/CD50
• Identification du gène (NCBI): 3385
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ICAM3 (Intercellular adhesion molecule 3) is also named as CD50, CDw50 and ICAM-R. ICAM3 is a member of the intercellular adhesion molecule (ICAM) family. ICAM3, being expressed constitutively on T cells and other leukocytes, is a potent signalling molecule and a major ligand in the initiation of T cell-mediated immune responses (PMID: 1448173, PMID: 11812993). ICAM3 is the natural ligand for both DC-SIGN and L-SIGN (PMID: 17570115). ICAM3 is abundantly and constitutively expressed in all leukocytes and is probably the most important ligand for LFA-1 in initiating immune responses, and ICAM3 is a typical type I transmembrane glycoprotein (PMID: 39991572). The promoter region of ICAM3 can be bound by co-transcription factors such as ETs and C/EBP, promoting macrophage maturation as well as monocyte extravasation (PMID: 22479382). ICAM3 is widely expressed not only in lymphocytes such as T and B cells but also in many endothelial cells and dendritic cells (PMID: 39991572).

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