Anticorps Recombinant de lapin anti-ICAM3/CD50

• Phare
• Validé par KD/KO

ICAM3/CD50 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

241417F3

N° de cat : 84335-5-RR

Synonymes

CDW50, ICAM3, 241417F3, CD50, ICAM 3

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Galerie de données de validation

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  WB analysis using 84335-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84335-5-RR (ICAM3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 84335-5-RR

  WB result of ICAM3/CD50 antibody (84335-5-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ICAM3/CD50 transfected Jurkat cells.

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  Affinity and Kinetic Characterization of 84335-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84335-5-RR against Human ICAM3/CD50 were performed. The affinity constant is 24.7 nM.

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules Ramos, cellules U-937

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84335-5-RR cible ICAM3/CD50 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: intercellular adhesion molecule 3
• Masse moléculaire calculée: 547 aa, 60 kDa
• Poids moléculaire observé: 110-130 kDa
• Numéro d’acquisition GenBank: BC058903
• Symbole du gène: ICAM3/CD50
• Identification du gène (NCBI): 3385
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Intercellular Adhesion Molecule 3 (ICAM-3), also known as CD50, is a member of the immunoglobulin superfamily. It is a transmembrane glycoprotein that contains an N-terminus extracellular domain, a single transmembrane domain, and a C-terminus cytoplasmic domain. ICAM-3 possesses 2-9 Ig-like C2-type domains in its extracellular region and is known to bind to the leukocyte adhesion protein LFA-1 . This molecule is expressed on leukocytes and epidermal Langerhans cells and plays a significant role in immune cell interactions and T lymphocyte activation.

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