Anticorps Recombinant de lapin anti-ICAM3/CD50

ICAM3/CD50 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC

Conjugaison

Non conjugué

CloneNo.

241418E8

N° de cat : 98097-1-RR

Synonymes

CDW50, ICAM3, 241418E8, CD50, ICAM 3

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Galerie de données de validation

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  FC experiment of human PBMCs using 98097-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human ICAM3/CD50 Rabbit Recombinant Antibody (98097-1-RR, Clone:241418E8) or Isotype Control and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human PBMCs using 98097-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human ICAM3/CD50 Rabbit Recombinant Antibody (98097-1-RR, Clone:241418E8) or Isotype Control and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human PBMCs using 98097-1-RR

  1x10^6 FC receptor blocked human PBMCs were surface stained with 0.25 ug Anti-Human ICAM3/CD50 Rabbit Recombinant Antibody (98097-1-RR, Clone:241418E8) and PE-conjugated Goat Anti-Rabbit IgG, and 0.25 ug Isotype Control. Cells were not fixed.

• 

  Affinity and Kinetic Characterization of 98097-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98097-1-RR against Human ICAM3/CD50 were performed. The affinity constant is 0.366 nM.

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Applications testées

• Résultats positifs en cytométrie: CMSP humaines

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98097-1-RR cible ICAM3/CD50 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: intercellular adhesion molecule 3
• Masse moléculaire calculée: 547 aa, 60 kDa
• Numéro d’acquisition GenBank: BC058903
• Symbole du gène: ICAM3/CD50
• Identification du gène (NCBI): 3385
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2 - 8°C. Stable for one year after shipment.

Informations générales

The intercellular adhesion molecule (ICAM) family is a subfamily of immunoglobulin superfamily with five known ICAM family members (ICAM1 to ICAM5) which play a role in inflammation, immune responses, and intracellular signaling (PMID: 23287428; 29477378). ICAM3, also known as CD50, is a type I transmembrane glycoprotein that contains five C2-type immunoglobulin-like domains in its extracellular region, a single transmembrane segment, and a C-terminus cytoplasmic tail. ICAM3 is selectively expressed on leukocytes (PMID: 1730916; 8132828). It is the primary ligand for LFA-1 and is involved in various immune processes, including T-cell activation, and macrophage recognition of apoptotic cells (PMID: 11812993; 23775590).

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