Anticorps Recombinant de lapin anti-ICOS/CD278

ICOS/CD278 Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242639D5

N° de cat : 85035-2-PBS

Synonymes

242639D5, Activation-inducible lymphocyte immunomediatory molecule, Ailim, CCLP, CD278

---

Galerie de données de validation

• 

  WB analysis using 85035-2-RR (same clone as 85035-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85035-2-RR (ICOS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85035-2-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 85035-2-RR (same clone as 85035-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 85035-2-RR (ICOS/CD278 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85035-2-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 85035-2-RR (same clone as 85035-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 85035-2-RR (ICOS/CD278 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85035-2-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP01745-2

  Cytometric bead array standard curve of MP01745-2, MOUSE ICOS Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85035-2-PBS. Detection antibody: 85035-3-PBS. Standard: Eg3198. Range: 0.625-80 ng/mL.

---

Informations sur le produit

85035-2-PBS cible ICOS/CD278 dans les applications de WB, IHC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ICOS/CD278 Protéine recombinante Eg3198
• Nom complet: inducible T-cell co-stimulator
• Masse moléculaire calculée: 23kDa
• Poids moléculaire observé: 23 kDa
• Numéro d’acquisition GenBank: NM_017480.2
• Symbole du gène: Icos
• Identification du gène (NCBI): 54167
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Inducible T cell co-stimulator (ICOS) is related to the CD28 superfamily and is highly expressed on activated T cells as well as regulatory T cells and is crucial for the survival and function of T cells, Th2 cell differentiation, and lung inflammatory responses. Binding ICOS to ICOS-ligand (ICOS-L) activates a cascade of intracellular signaling molecules that prevent apoptosis and lead to the production of cytokines such as IL-4 and IL-13.

{{ptg:RelatedPrimaryAntibodies}}