Anticorps Recombinant de lapin anti-ICOS/CD278

ICOS/CD278 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

FC

Conjugaison

Biotin

CloneNo.

242639C3

N° de cat : Biotin-FcA98341

Synonymes

Activation-inducible lymphocyte immunomediatory molecule, Ailim, CCLP, CD278, CD28 and CTLA-4-like protein

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Galerie de données de validation

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  FC experiment of BALB/c spleen cells using Biotin-FcA98341

  1x10^6 untreated or anti-CD3/CD28 treated BALB/c spleen cells were surface stained with 0.25 ug Biotin Anti-Mouse ICOS/CD278 Rabbit RecAb (Biotin-FcA98341, Clone: 242639C3) and PE Streptavidin(Cat.No. PF00021). Cells were co-stained with FcZero-rAb™ APC Anti-Mouse CD3e (145-2C11) Rabbit IgG Recombinant Antibody (APC-FcA65651, Clone: 145-2C11). Cells were not fixed.

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  FC experiment of BALB/c spleen cells using Biotin-FcA98341

  1x10^6 anti-CD3/CD28 treated BALB/c spleen cells were surface stained with 0.25 ug Biotin Anti-Mouse ICOS/CD278 Rabbit RecAb (Biotin-FcA98341, Clone: 242639C3) (red) or or FcZero-rAb™ Biotin Rabbit IgG Isotype Control Recombinant Antibody (Biotin-FcA98136, Clone: 240953C9) (blue), and PE Streptavidin(Cat.No. PF00021). 1x10^6 BALB/c spleen cells were surface stained with 0.25 ug Biotin Anti-Mouse ICOS/CD278 Rabbit RecAb (Biotin-FcA98341, Clone:242639C3) (black, dashed) and PE Streptavidin(Cat.No. PF00021). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: Anti-CD3/CD28 treated BALB/c spleen cells

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

Biotin-FcA98341 cible ICOS/CD278 dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ICOS/CD278 Protéine recombinante Eg3198
• Nom complet: inducible T-cell co-stimulator
• Masse moléculaire calculée: 23kDa
• Numéro d’acquisition GenBank: NM_017480.2
• Symbole du gène: Icos
• Identification du gène (NCBI): 54167
• Conjugaison: Biotin
• Excitation/Emission maxima wavelengths: -
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Inducible T cell co-stimulator (ICOS) is related to the CD28 superfamily and is highly expressed on activated T cells as well as regulatory T cells and is crucial for the survival and function of T cells, Th2 cell differentiation, and lung inflammatory responses. Binding ICOS to ICOS-ligand (ICOS-L) activates a cascade of intracellular signaling molecules that prevent apoptosis and lead to the production of cytokines such as IL-4 and IL-13.

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