Anticorps Monoclonal anti-ICOSLG/B7-H2/CD275

ICOSLG/B7-H2/CD275 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

2G1F8

N° de cat : 67003-1-Ig

Synonymes

B7H2, ICOSLG, B7RP1, B7-related protein 1, B7-like protein Gl50

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Galerie de données de validation

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  WB analysis using 67003-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67003-1-Ig (ICOSLG antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of Daudi using 67003-1-Ig

  Daudi cells were subjected to SDS PAGE followed by western blot with 67003-1-Ig (ICOSLG antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HL-60, cellules Daudi, cellules HUVEC, cellules JAR

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

67003-1-Ig cible ICOSLG/B7-H2/CD275 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ICOSLG/B7-H2/CD275 Protéine recombinante Ag6799
• Nom complet: inducible T-cell co-stimulator ligand
• Masse moléculaire calculée: 33 kDa, 34 kDa
• Poids moléculaire observé: 60-65 kDa, 28-35 kDa
• Numéro d’acquisition GenBank: BC064637
• Symbole du gène: ICOSLG
• Identification du gène (NCBI): 23308
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ICOSLG, also known as B7H2 or GL50, is a type I transmembrane glycoprotein belonging to the B7 ligand family. ICOSLG is extensively expressed on professional antigen-presenting cells including B cells, macrophages, and dendritic cells, as well as non-lymphoid cells including mesenchymal stem cells, endothelial cells, fibroblasts, and tumor cells (PMID: 33756276; 35800767). It is a specific ligand for the T-cell-specific cell surface receptor ICOS and acts as a co-stimulatory molecule (PMID: 11023515; 11007762). The interaction of ICOSLG and ICOS plays important roles in the activation, proliferation, differentiation, and cytokine production of T cells as well as in the antibody secretion from B cells during secondary immune responses (PMID: 21851236).

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