Anticorps Recombinant de lapin anti-ICOSLG/B7-H2/CD275

ICOSLG/B7-H2/CD275 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

FC

Conjugaison

Non conjugué

CloneNo.

240243G5

N° de cat : 98058-1-PBS

Synonymes

CD275, B7-H2, Icosl, ICOS L, GL50

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Galerie de données de validation

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  FC experiment of mouse splenocytes using 98058-1-RR (same clone as 98058-1-PBS)

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse ICOSLG/B7-H2/CD275 Rabbit Recombinant Antibody (98058-1-RR, Clone:240243G5) or Isotype Control and PE-conjugated Goat Anti-Rabbit IgG. Cells were then stained with CoraLite® Plus 647 Anti-Mouse CD45R (B220). Cells were not fixed. This data was developed using the same antibody clone with 98058-1-PBS in a different storage buffer formulation.

• 

  FC experiment of mouse splenocytes using 98058-1-RR (same clone as 98058-1-PBS)

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse ICOSLG/B7-H2/CD275 Rabbit Recombinant Antibody (98058-1-RR, Clone:240243G5) (red) or Isotype Control (blue) and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed. This data was developed using the same antibody clone with 98058-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98058-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98058-1-RR against Mouse ICOSLG/B7-H2/CD275 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

98058-1-PBS cible ICOSLG/B7-H2/CD275 dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: icos ligand
• Masse moléculaire calculée: 36 kDa
• Numéro d’acquisition GenBank: NM_015790.3
• Symbole du gène: CD275
• Identification du gène (NCBI): 50723
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C.

Informations générales

ICOSLG, also known as B7H2 or GL50, is a type I transmembrane glycoprotein belonging to the B7 ligand family. ICOSLG is extensively expressed on professional antigen-presenting cells including B cells, macrophages, and dendritic cells, as well as non-lymphoid cells including mesenchymal stem cells, endothelial cells, fibroblasts, and tumor cells (PMID: 33756276; 35800767). It is a specific ligand for the T-cell-specific cell surface receptor ICOS and acts as a co-stimulatory molecule (PMID: 11023515; 11007762). The interaction of ICOSLG and ICOS plays important roles in the activation, proliferation, differentiation, and cytokine production of T cells as well as in the antibody secretion from B cells during secondary immune responses (PMID: 21851236).

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