Anticorps Recombinant de lapin anti-ICOSLG/B7-H2/CD275

ICOSLG/B7-H2/CD275 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

FC

Conjugaison

Non conjugué

CloneNo.

240243G5

N° de cat : 98058-1-RR

Synonymes

CD275, B7-H2, Icosl, ICOS L, GL50

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Galerie de données de validation

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  FC experiment of mouse splenocytes using 98058-1-RR

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse ICOSLG/B7-H2/CD275 Rabbit Recombinant Antibody (98058-1-RR, Clone:240243G5) or Isotype Control and PE-conjugated Goat Anti-Rabbit IgG. Cells were then stained with CoraLite® Plus 647 Anti-Mouse CD45R (B220). Cells were not fixed.

• 

  FC experiment of mouse splenocytes using 98058-1-RR

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse ICOSLG/B7-H2/CD275 Rabbit Recombinant Antibody (98058-1-RR, Clone:240243G5) (red) or Isotype Control (blue) and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed.

• 

  Affinity and Kinetic Characterization of 98058-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98058-1-RR against Mouse ICOSLG/B7-H2/CD275 were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en cytométrie: splénocytes de souris,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98058-1-RR cible ICOSLG/B7-H2/CD275 dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: icos ligand
• Masse moléculaire calculée: 36 kDa
• Numéro d’acquisition GenBank: NM_015790.3
• Symbole du gène: CD275
• Identification du gène (NCBI): 50723
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2 - 8°C. Stable for one year after shipment.

Informations générales

ICOSLG, also known as B7H2 or GL50, is a type I transmembrane glycoprotein belonging to the B7 ligand family. ICOSLG is extensively expressed on professional antigen-presenting cells including B cells, macrophages, and dendritic cells, as well as non-lymphoid cells including mesenchymal stem cells, endothelial cells, fibroblasts, and tumor cells (PMID: 33756276; 35800767). It is a specific ligand for the T-cell-specific cell surface receptor ICOS and acts as a co-stimulatory molecule (PMID: 11023515; 11007762). The interaction of ICOSLG and ICOS plays important roles in the activation, proliferation, differentiation, and cytokine production of T cells as well as in the antibody secretion from B cells during secondary immune responses (PMID: 21851236).

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